黄精多糖与黄酮综合提取工艺优化及硒肥对其含量的影响  

Optimization of Extraction Technology of Polysaccharides and Flavonoids from Polygonatum sibiricum and Effects of Selenium Fertilizer on Its Content

作　　者：滕树锐 廖璐婧[3] 武芸[1] 江念[1,2] 郑小江 TENG Shu-rui;LIAO Lu-jing;WU Yun;JIANG Nian;ZHENG Xiao-Jiang(Gollcgc of Forestry Sciences and Horticulture,Hubei Institute for Nationalities,Enslii 445000,Hubei,China;Enslii Dadi Bioteehnology Research Institute,Enslii 445000,Hubei,China;Institutc of Traditional Chincsc Medicine,Hubei Academy of Agricultural Sciences,Enslii 445000,Hubei,China)

机构地区：[1]湖北民族学院林学园艺学院,湖北恩施445000 [2]恩施州大地生物科技研究所,湖北恩施445000 [3]湖北省农业科学院中药材研究所,湖北恩施445000

出　　处：《湖北农业科学》2017年第23期4572-4576,共5页Hubei Agricultural Sciences

基　　金：生物资源保护与利用湖北省重点实验室开放基金项目(PKLHB1525);湖北省科技计划研究与开发项目(2011BBB061);湖北省自然科学基金创新群体项目(2009CDA115)

摘　　要：采用水提醇沉法优化黄精(Polygonatum sibiricum)多糖与黄酮的综合提取工艺,通过调节不同的硒浓度,分析不同外源硒条件下黄精多糖与黄酮的含量。结果表明,黄精多糖与黄酮最佳综合提取工艺为料液比1∶70(g∶mL)、提取温度70℃、提取时间1.5 h、微波辅助时间2.5 min,在此工艺条件下,多糖得率186.35 mg/g,黄酮得率为69.54 mg/g。通过调节不同外源硒的浓度,得出25 mg/kg为黄精富集多糖和黄酮的最佳外源硒浓度。The extraction process of Polygonatum Polysaccharide and Polygonatum flavonoids was optimized by water extraction and alcohol precipitation.The content of polysaccharides and flavonoids in Polygonatum was obtained by adjusting different selenium concentrations.The results showed that Polygonatum polysaccharide and Polygonatum flavonoids of optimal synthesis process is:solid-liquid ratio l:70(g:m L),extraction temperature70,extraction time1.5h,microwave time2.5min,under this condition,the rate of186.35mg/g polysaccharides,flavonoids was69.54mg/g.By adjusting the concentration of different exogenous selenium,it was concluded that25mg/kg was the best exogenous selenium concentration for polysaccharides and flavonoids from Polygonatum.

关 键 词：黄精(Polygnatum sibirieum) 多糖 黄酮 综合提取 硒 

分 类 号：R282[医药卫生—中药学]