响应面法优化绿僵菌产绿僵菌素A的培养条件  

Optimization of culture condition of Metarhizium anisopliae for destruxin A production by response surface methodology

作　　者：张功营[1] 杨华[1] 董丽红[1] 万树青[1] ZHANG Gongying;YANG Hua;DONG Lihong;WAN Shuqing(College of Agriculture, South China Agricultural University/Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, Guangzhou 510642, China)

机构地区：[1]华南农业大学农学院/天然农药与化学生物学教育部重点实验室,广东广州510642

出　　处：《华南农业大学学报》2018年第1期76-82,共7页Journal of South China Agricultural University

基　　金：广东省烟草专卖局(公司)科技项目(粤烟科[2015]7号)

摘　　要：【目的】优化绿僵菌Metarhizium anisopliae产绿僵菌素A的培养条件,提高绿僵菌素的产量。【方法】应用响应面法设计试验,以Plackett-Burman试验设计和中心组合试验设计筛选得到影响绿僵菌液体发酵的关键因素。【结果】试验得到1个拟合程度高、误差小的模型,该模型给出的最佳培养条件为:蔗糖22.7 g·L^(–1)、蛋白胨13.4 g·L^(–1)和发酵时间9.70 d,预测绿僵菌素A最大质量浓度为6.90μg·mL^(–1),实际测得质量浓度为6.89μg·mL^(–1)。【结论】结果可为绿僵菌大规模发酵获得粗毒素提供理论依据,使绿僵菌粗毒素的广泛开发与应用成为可能。【Objective】To raise the destruxin A production of Metarhizium anisopliae by optimizing culture conditions of liquid fermentation.【Method】Experiments were designed by response surface methodology(RSM).The key factors affecting the liquid fermentation were screened by Plackett-Burman design and central composite design.【Result】A model with high fitting degree and small error was obtained.The optimum culture condition of this model was22.7g·L–1sucrose,13.4g·L–1peptone and9.70d for fermentation.The maximum predicted concentration of destruxin A was6.90μg·mL–1,while the actual measured concentration was6.89μg·mL–1.【Conclusion】The results can provide a theoretical basis for large-scale fermentation of M.anisopliae and production of crude toxin,which can potentially enable the widespread development and application of crude toxin.

关 键 词：绿僵菌 绿僵菌素 产量 响应面法 液体发酵 培养条件 

分 类 号：S476[农业科学—农业昆虫与害虫防治]