激酶检测方法的最新研究进展  

Recent Advances in Sensitive Detection of Kinases

作　　者：刘杨 邹笑然 李琛琛 张春阳[1] LIU Yang;ZOU Xiao-Ran;LI Chen-Chen;ZHANG Chun-Yang(College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, China)

机构地区：[1]山东师范大学化学化工与材料科学学院,济南250014

出　　处：《分析化学》2018年第1期11-19,共9页Chinese Journal of Analytical Chemistry

基　　金：国家杰出青年科学基金项目(No.21325523);科技部重点领域创新团队项目(No.2015RA4024)资助~~

摘　　要：激酶是生物体内一类重要的磷酸转移酶,能够催化磷酸基团由高能磷酸基团供体分子(如ATP)向特定底物分子转移。激酶不但在新陈代谢、细胞信号传导、蛋白质调控、细胞传输等过程中起着关键作用,而且在临床诊断、药物研发及疾病靶向治疗等方面也发挥着重要作用。因此,发展灵敏度高、特异性好的激酶检测方法十分必要。本文以蛋白激酶A(Protein kinase A,PKA)、酪蛋白激酶(Casein kinase-2,CKII)、T4多核苷酸激酶(T4 polynucleotide kinase,T4 PNK)为例,对近年来发展的激酶检测方法进行了综述,着重介绍了荧光分析法、单分子检测、比色法、化学发光和生物发光分析法、电化学和光电化学分析法,并对激酶检测方法的发展方向进行了展望。Kinases are a class of enzymes that catalyze the transfer of a phosphate group from a high-energy molecule to their substrates(i.e.,phosphorylation).Kinase-induced intracellular phosphorylation plays important roles in a variety of cellular processes including metabolism,cell signaling,protein regulation,DNA replication and repair.Consequently,kinases have become potential biomarkers for disease diagnosis and drug discovery,and the development of simple and sensitive methods for kinase assay is highly desirable.In this review,we summarize the recent advances in kinase assays with protein kinase A(PKA),casein kinase-2(CKII)and T4polynucleotide kinase(T4PNK)as the models.We focus on the newly emerging methods for kinase assays including fluorescent,single-molecule detection,colorimetric,chemiluminescent,bioluminescent,and electrochemical and photoelectrochemical methods.Furthermore,we give a new insight into the future direction of kinase assays.

关 键 词：激酶 灵敏检测 分析方法 评述 

分 类 号：O652[理学—分析化学] Q55[理学—化学]