一种基于香豆素衍生物的铁离子水溶性荧光探针的合成及其应用  

Synthesis and Biological Application of A Water-Soluble Fluorescent Probe Based on Coumarin Derivatives for Detection of Ferric Ion

作　　者：陈兆辉 李媛媛 韩娟[3] 王赟[1] 任彦鹏 陈桐[4] 唐旭[1] 倪良[1] CHEN Zhao-Hui;LI Yuan-Yuan;HAN Juan;WANG Yun;REN Yan-Peng;CHEN Tong;TANG Xu;NI Liang(School of Chemistry and Chemical Engineering Jiangsu University, Zhenjiang 212013, China;Jingjiang college Jiangsu University, Zhenjiang 212013, China;School of Food and Biological Engineering Jiangsu University, Zhenjiang 212013, China;Zhenjiang Entry-exit Inspection and Quarantine Bureau, Zhenjiang 212000, China)

机构地区：[1]江苏大学化学化工学院,镇江212013 [2]江苏大学京江学院,镇江212013 [3]江苏大学食品与生物工程学院,镇江212013 [4]镇江出入境检验检疫局,镇江212000

出　　处：《分析化学》2018年第1期20-26,共7页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(No.21576124);江苏出入境检验检疫局科技计划项目(No.2016KJ51);镇江市重点研发计划--社会发展项目(Nos.SH2015014;SH2016019)资助~~

摘　　要：设计合成了一种基于香豆素衍生物的水溶性荧光探针7-二乙氨基-3-甲醛香豆素,并通过~1HNMR,^(13)CNMR和MS确认其结构。探针具有良好的荧光发射性能,荧光最大发射峰位于471 nm;向其中加入Fe^(3+)后,荧光强度随着Fe^(3+)浓度的增加而逐渐减弱。探针L的荧光发射强度与Fe^(3+)的浓度在0.02~60.00μmol/L范围内呈良好的线性关系,可对Fe^(3+)进行定性与定量检测,检出限为22 nmol/L(S/N=3),对Fe^(3+)的选择性良好,不受其它常见金属离子的干扰。此外,探针对Fe^(3+)的检测具有可逆性。本探针具有很好的水溶性,在生理p H环境中检测效果较好成功用于人体淋巴肿瘤细胞Ramos中Fe^(3+)荧光成像。A water-soluble fluorescent probe(7-diethyl amino-3-formaldehyde coumarin)for Fe3+detection was designed and synthesized,and its structure was confirmed by1HNMR,13CNMR and MS spectra.This probe showed high emission intensity at471nm.With the continuous addition of Fe3+,the emission intensities at471nm decreased gradually,and showed an excellent linearity with Fe3+in the range of0.02-60滋mol/L,and the regression equation was I=322.56-4.86CFe3+.This probe was able to detect Fe3+qualitatively and quantitatively with the detection limit as low as22nmol/L.Besides,this probe showed high sensitivity to Fe3+over other metal ions.The detection process was reversible,which could be recycled for the Fe3+detection.In terms of good optical properties and the strong fluorescence in physiological pH,the probe was successfully applied in imaging Fe3+of living Ramos cells.

关 键 词：荧光探针 铁离子 水溶性 细胞成像 

分 类 号：O657.3[理学—分析化学] R446.1[理学—化学]