三聚氰胺分子印迹表面等离子体共振传感器的制备及分析应用  

Preparation of Melamine Molecularly Imprinted Surface Plasmon Resonance Sensor and Its Analytical Application

作　　者：张丽丽[1] 李洁[1] 陈长宝[1] 朱树华[1] 周杰[1] 尚鹏鹏[1] ZHANG Li-Li;LI Jie;CHEN Chang-Bao;ZHU Shu-Hua;ZHOU Jie;SHANG Peng-Peng(College of Chemistry and Materials Science, Shandong Agricultural University, Taian 271018, China)

机构地区：[1]山东农业大学化学与材料科学学院,泰安271018

出　　处：《分析化学》2018年第1期88-93,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.31370686;31470686)资助~~

摘　　要：以合成的烯丙基-1,3,5-三嗪-2,4,6-(1H,3H,5H)三酮为功能单体,三聚氰胺为模板分子,乙二醇二甲基丙烯酸酯为交联剂,在表面等离子体共振(SPR)传感芯片上合成了分子印迹聚合物薄膜。根据印迹聚合物的朗格缪尔方程式,计算得到三聚氰胺和分子印迹聚合物之间的结合常数为1.40×10~8L/mol。此传感器显示出对模板分子三聚氰胺的高选择性,在p H=7.5的条件下,此传感器对三聚氰胺的响应最佳,线性范围为0.1-10.0 nmol/L(R=0.9991)。将此传感器用于牛奶和奶粉中三聚氰胺的检测,检出限分别为62.6 pmol/L和56.4 pmol/L(S/N=3),回收率分别为91.5%和92.0%。The melamine molecular imprinted polymer(MIP)membrane immobilized on the surface of the surface plasmon resonance(SPR)chip was prepared with allylcyanurate and ethylene glycol dimethacrylate as a functional monomer and cross-linker,respectively.Based on the ratio of the intercept to the slope of Langmuir equation for imprinted polymer,the association constant(Kass)of imprinted sites in the imprinted film to melamine was calculated to be1.40×108L/mol.The SPR sensor showed high selectivity to the template molecule melamine,and the best response was obtained at pH=7.5.The method showed good linearity in the melamine concentration range of0.1-10.0nmol/L(R=0.9991).Based on a signal to noise ratio of3,the detection limits of melamine were62.6pmol/L and56.4pmol/L for the milk and milk powder,respectively.The recoveries were91.5%for milk and92.0%for milk powder.These results suggest that SPR sensing combined with MIP film is a promising alternative method for detection of melamine.

关 键 词：三聚氰胺 分子印迹 表面等离子体共振传感器 

分 类 号：O631.3[理学—高分子化学] TP212[理学—化学]