α-细辛醚对食管癌Eca-109细胞线粒体凋亡通路的影响  

Effect of α-Asarone on Esophageal Eca-109 Cell Mitochondrial Apoptosis Pathway

作　　者：王白燕[1] 张方方[1] 韩倩倩[1] 李瑞琴[2] 朱艳琴[1] WANG Baiyan;ZHANG Fangfang;HAN Qianqian;LI Ruiqin;ZHU Yanqin(Experimental Teaching Center,Basic Medical School,Zhengzhou 450046,China;Pathology Center,Henan College of Traditional Chinese Medicine,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学基础医学院实验教学中心,郑州450046 [2]河南中医药大学病理实验中心,郑州450046

出　　处：《医药导报》2018年第1期27-30,共4页Herald of Medicine

基　　金：郑州市科技攻关项目(131PPTGG417-1);河南省高等学校重点科研项目(16A310021);河南省中医药科学研究专项课题(2016ZY2017)

摘　　要：目的探讨α-细辛醚对人食管癌Eca-109细胞线粒体凋亡通路Cyt-c、Smac及Caspase3 mRNA和蛋白表达水平的影响。方法体外培养的人食管癌Eca-109细胞设为阴性对照组和α-细辛醚组,α-细辛醚终浓度分别为25,50,100μg·m L^(-1),培养48 h后使用荧光倒置显微镜观察细胞凋亡形态改变,采用TRIzol法提取细胞总RNA,实时荧光定量聚合酶链反应检测细胞Cyt-c、Smac及Caspase3 mRNA的表达,Western blotting检测其蛋白表达,选取β-actin进行相对定量分析。结果α-细辛醚组细胞形态发生明显凋亡改变;与阴性对照组比较,α-细辛醚组细胞Cyt-c、Smac及Caspase3mRNA和蛋白的表达量均明显增加(P<0.05)。结论α-细辛醚可通过调节线粒体凋亡途径相关基因Cyt-c、Smac及Caspase3的表达增加诱导Eca-109细胞凋亡。ObjectiveTo discuss the effect ofα-asarone on the expression level of Cyt-c,Smac,Caspase3mRNA and protein in human esophageal carcinoma Eca109cell mitochondria.MethodsThe Eca-109cells were cultured in vitro,and divided into the negative control group and theα-asarone treatment groups(final concentration:25,50,100μg·mL-1).After48h,the morphological changes of Eca109cells were observed by fluorescence inversion microscope.The total RNA of cells were extracted by TRIzol method,the expressions of Cyt-c、Smac and Caspase3were measured by RT PCR and Western blotting.ResultsAfter Eca109cells were treated with different concentrations ofα-asarone for48h,and obvious changes in the morphology were observed,the expressions of Cyt-c,Smac and Caspase3genes and protein were increased significantly compared to the negative control group(P<0.05).Conclusionαasarone can induce the human Eca-109cells apoptosis by regulating expressions of mitochondrial apoptosis pathway correlation genes such as Cyt-c,Smac and Caspase3.

关 键 词：Α-细辛醚 Eca-109细胞线粒体 癌 食管 CYT-C SMAC CASPASE3 

分 类 号：R282.71[医药卫生—中药学] R965[医药卫生—中医学]