脐带间质干细胞来源外泌体的安全性评估  

Security evaluation of exosome derived from human umbilical cord mesenchymal stem cells

作　　者：骆峻 丁利军[2] 周强[3] 韩奕文 缪文玲 LUO Jun;DING Lijun;ZHOU Qiang;HAN Yiwen;MIAO Wenling(Central Laboratory,Jiangsu Health Vocational College,Nanjing,Jiangsu 210029,China;Reproductive Medicine Center,Nanjing Drum Tower The Affiliated Hospital of Nanjing University Medical School,Nanjing,Jiangsu 210008,China;Department of Pathology,Nanjing Drum Tower The Affiliated Hospital of Nanjing University Medical School,Nanjing,Jiangsu 210008,China)

机构地区：[1]江苏卫生健康职业学院中心实验室,南京210029 [2]南京大学医学院附属鼓楼医院生殖中心,南京210008 [3]南京大学医学院附属鼓楼医院病理科,南京210008

出　　处：《国际检验医学杂志》2017年第24期3371-3373,3376,共4页International Journal of Laboratory Medicine

基　　金：国家自然科学基金资助项目(81571391)

摘　　要：目的评估脐带间质干细胞(huc-MSC)来源的外泌体作为一种临床应用生物制品的自身安全性。方法超速离心法分离外泌体,利用蛋白质印迹法(WB)检测外泌体表面标记CD9、CD63,利用纳米颗粒跟踪分析技术(NTA)检测外泌体的大小及浓度。溶血试验、全身过敏性试验和血常规检测验证外泌体的自身安全性。结果外泌体表达外泌体特异性分子CD9、CD63,外泌体不会引起溶血、全身过敏反应及血常规异常。结论脐带间质干细胞来源的外泌体具有外泌体的一般特征,且具有一定的安全性,为外泌体今后在临床上的应用提供了实验依据。Objective To explore the security of exosome derived from human umbilical cord mesenchymal stem cell(hucMSCexosome)as a kind of biological product on clinical application.Methods hucMSCexosome were separated by ultea centrifuge.CD9and CD63expression of hucMSCexosome were tested by Westernblot.Nanoparticle analysis was used to detect the size and concentration of hucMSCexosome.Hemolytic experiment,systemic anaphylaxis and blood routine test were used to verify the selfsafety of hucMSCexosome.Result shucMSCexosome expressed specific markers of exosome,CD9and CD63.Moreover,hucMSCexosome cannot result in hemolysis,systemic anaphylaxis reaction and abnormality of blood routine test.Conclusion Exosome derived from human umbilical cord mesenchymal stem cell has general signs of exosomes and possesses the certainly security,which may provide the experimental evidence for hucMSCexosome clinical application.

关 键 词：脐带间质干细胞 外泌体 安全性 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]