Nano DSC法测定ILPR G-四链体的热力学性质  

Study on Thermodynamic Parameters of Insulin Linkage Polymorohism Region G-Quadruplex by Using A Nano Differential Scanning Calorimetry

作　　者：张又右 史全[2] 王思雨[1] 谭志诚[2] 王韶旭[1] ZHANG Youyou;SHI Quan;WANG Siyu;TAN Zhicheng;WANG Shaoxu(School of Environmental and Chemical Engineering,Dalian Jiaotong University,Dalian 116028, China;Dalian Institute of Chemical Physics,Chinese Academy of Sciences, Dalian 116023,China)

机构地区：[1]大连交通大学环境与化学工程学院,辽宁大连116028 [2]中国科学院大连化学物理研究所热化学实验室,辽宁大连116023

出　　处：《大连交通大学学报》2018年第1期100-104,共5页Journal of Dalian Jiaotong University

基　　金：辽宁省自然科学基金资助项目(2015020211)

摘　　要：采用差示扫描微量热仪(Nano DSC)研究了人工合成的胰岛素连锁多肽区(insulin linkage polymorohism region,ILPR)DNA片段在钾离子溶液中形成的G-四链体的热力学性质.在40~100℃温区准确测量了G-四链体的熔融温度、融化焓和等压比热.结果表明,ILPR G-四链体的构象转变的熔融中点温度在(67.63±0.16)℃和(96.2±0.01)℃,两个分立组份的融化焓分别为(170.9±3.7)kJ/mol和(392.0±0.75)kJ/mol.The thermodynamic properties of the insulin linkage polymorohism region(ILPR)G quadruplex in the presence of K+ions were measured by nano differential scanning calorimetry(Nano DSC)in solution.The melting temperature melting enthalpy and constant pressure specific heat of the G quadruplex were measured accurately in the range of40℃to110℃.The DSC thermogram of this sequence shows a melting curve with melting transitions of(67.63±0.02)℃and(96.2±0.02)℃,and the melting enthalpies are(170.9±2.1)kJ/mol and(392.0±1.6)kJ/mol.

关 键 词：G-四链体 ILPR 热力学参数 等压比热 

分 类 号：Q52[生物学—生物化学]