碳饥饿和盐胁迫处理过表达OsAtg8a水稻转化植株的基因表达分析  

Gene expression analysis of overexpression OsAtg8a transgenic rice plants under carbon starvation and salt stress

作　　者：吴兴超 陈喜红 杜雪竹[1] 居超明[1] WU Xingchao;CHEN Xihong;DU Xuezhu;JU Chaoming(College of Life Sciences,Hubei University,Wuhan 430062,China)

机构地区：[1]湖北大学生命科学学院,湖北武汉430062

出　　处：《湖北大学学报（自然科学版）》2018年第1期12-17,共6页Journal of Hubei University：Natural Science

基　　金：优质;高产;广适;多抗籼稻新品种培育(2016ABA090)项目资助

摘　　要：为了探究Os Atg8a在水稻碳饥饿和盐胁迫过程中的作用,通过农杆菌介导法获得了过表达Os Atg8a的水稻转化植株.分别对野生型植株和转基因植株进行碳源饥饿处理0、8、16、24和48 h,发现碳饥饿处理时间为0、8、16、24 h时野生型植株和转基因植株中基因表达量均没有发生明显变化,处理48 h时,过表达Os Atg8a植株的Os Atg1a、Os Atg4a、Os Atg8a、Os Atg13a、Os Atg16a和Os TOR基因表达量均显著高于对照植株.盐胁迫处理组合下,发现250 mmol/L Na Cl处理4 h的过表达Os Atg8a植株中,Os Atg8a和Os Atg16a的表达量较未处理的转基因植株表达量明显增加,Os Atg8a的表达量较野生型增加了36倍.针对过表达Os Atg8a转化植株在无碳源和高盐胁迫过程中相关自噬基因表达量明显升高的结果,推测Os Atg8a基因在水稻抗非生物胁迫的过程中具有重要的作用.In order to explore the role of OsAtg8a in the process of carbon starvation and salt stress in rice,overexpression OsAtg8a plants were obtained by Agrobacterium mediated transformation.During the treatment of carbon starvation for0,8,16,24and48h,expression levels of OsAtg1a,OsAtg4a,OsAtg8a,OsAtg13a,OsAtg16a and OsTOR in the transgenic plants were significantly higher than that in the control at48h.Under the treatment of250mmol/L NaCl salt stress for4h,OsAtg8a and OsAtg16a expression levels of transgenetic plants were much higher than that of control.The expression of OsAtg8a was36times higher than that of wild type.The result of autophagy related genes expression increased significantly in the overexpression OsAtg8a transgenic plants under the conditions of carbon starvation and high salt concentration indicated that OsAtg8a gene plays an important role in rice abiotic stress resistance.

关 键 词：水稻 OsAtg8a 过表达 碳饥饿 盐胁迫 基因表达分析 

分 类 号：Q37[生物学—遗传学]