不同稀释度对凝血功能指标检测结果影响的实验研究  

An experiment study of the effect of different blood dilution state on blood coagulation function

作　　者：史连义[1] 周志伟[1] 王莎 陈双松 付振利 周嘉慧 程娜娜 SHI Lianyi;ZHOU Zhiwei;WANG Sha;CHEN Shuangsong;FU Zhenli;ZHOU Jiahui;CHENG Nana(Department of Clinical Laboratory and transfusion,the Central Hospital of China National Petroleum Corporation,Hebei Province,Langfang 065000,China)

机构地区：[1]中国石油天然气集团公司中心医院,廊坊065000 [2]廊坊市医学科学情报站,065000

出　　处：《国际检验医学杂志》2017年第A02期4-6,共3页International Journal of Laboratory Medicine

基　　金：廊坊市科技局科技支撑项目(2014013123).

摘　　要：目的探讨不同稀释度对凝血功能的影响,为临床根据失血量进行血浆输注提供实验依据。方法随机抽取40例凝血结果正常且未服用抗凝药患者的血浆,用0.9%NACL以10∶0(不稀释)、8∶2、6∶4、4∶6、2∶8的比例进行稀释,模拟失血情况,分别检测不同稀释度的PT、APTT、FIB,结果进行统计学分析。结果40例患者的PT、APTT、FIB不同稀释度时均有明显的变化(P<0.05),PT在4:6稀释时约为正常值的1.5倍(P<0.05),APTT在6:4稀释时约为正常值的1.5倍(P<0.05),APTT在4:6稀释时约为正常值的2.0倍(P<0.05),FIB在4:6稀释时约为1.0g/L(P<0.05)。结论当患者自体血液剩余低于40%之前(失血量60%),应及早补充血浆等凝血因子维持正常凝血功能。Objective To investigate the effect of different blood dilution state on blood coagulation function,and provide experiment evidence for plasma transfusion according to blood loss volume.Methods The plasma of40patients were chosen randomly,and diluted with0.9%NACL.The dilution ratios of plusma to0.9%NACL were10:0(undiluted)、8∶2、6∶4、4∶6、2∶8,PT、APTT、FIB of different dilutions were detected and analysised by spss.Results In40patients with PT、APTT、FIB changes with different dilutions were significantly(P<0.05).PT is1.5times longer at4∶6than10∶0,APTT is1.5times longer at6∶4than10∶0,and is2.0times longer at4∶6than10∶0,FIB is ahout1.0g/L at4∶6dilution.Conclusion For preserving normal blood coagulation function,fresh frozen plasma should be transfused when the residual content of coagulation factor in blood less than40%(blood loss is about60%).

关 键 词：稀释 凝血功能 凝血酶原时间 活化部分凝血活酶时间 血浆纤维蛋白原含量 

分 类 号：R654.1[医药卫生—外科学]