中药复方多糖对不同MHC B-LβⅡ基因型鸡淋巴细胞IL-2、IL-4、IL-12 mRNA表达量的影响  

Effects of Traditional Chinese Medicinal Polysaccharide Extraction on Chicken Lymphocyte of IL-2,IL-4 and IL-12 mRNA Expression in Different MHC B-LβⅡ Genotype Chickens Lymphocyte

作　　者：马昭[1] 连科讯 刘钢[1] 刘晓婷[1] 乔严杰 朱晓庆[1] 谷新利[1] MA Zhao;LIAN KE-xun;LIU Gang;LlU Xiao-ting;QIAO Yan-jie;ZHU Xiao-qing;GU Xin-li(College of Animal Science and Technology, Shihezi University, Shihezi Xinjiang 832003, China)

机构地区：[1]石河子大学动物科技学院,新疆石河子832003

出　　处：《新疆农业科学》2017年第12期2320-2328,共9页Xinjiang Agricultural Sciences

基　　金：地区科学基金项目“一定纯度中药复方多糖对不同MHC B-LβⅡ基因型鸡免疫调节作用及其作用机制研究”(31460673)~~

摘　　要：【目的】中药复方多糖对不同MHC B-LβⅡ基因型鸡淋巴细胞IL-2、IL-4、IL-12 mRNA表达量的影响。【方法】采用PCR-SSCP方法将500羽京红1号蛋鸡按不同MHC B-LβⅡ基因型分组,采集不同MHC B-LβⅡ基因型鸡外周血淋巴细胞,分别加入终浓度为100、75、50、0μg/mL中药复方多糖共培养24 h,收集细胞,采用Real-time PCR方法检测不同MHC B-LβⅡ基因型鸡IL-2、IL-4、IL-12基因mRNA的表达量。【结果】(1)与对照组相比,不同剂量中药复方多糖均能显著增强不同MHC B-LβⅡ基因型鸡IL-2、IL-4、IL-12基因mRNA的表达量(P<0.05)。(2)中药复方多糖剂量为50μg/mL时,AA、BC基因型鸡IL-2、IL-4、IL-12基因mRNA的表达量最高,中药复方多糖剂量为100μg/mL时,BB基因型鸡IL-2、IL-4、IL-12基因mRNA的表达量最高。【结论】中药复方多糖能不同程度的促进各MHC B-LβⅡ基因型鸡IL-2、IL-4、IL-12 mRNA的表达,且不同MHC B-LβⅡ基因型鸡淋巴细胞最适中药复方多糖免疫调节剂量不同。【Objective】To explore the effect of polysaccharide extracted from traditional Chinese medicines on immunomodulatory in different MHC B LβII genotype chickens.【Method】PCR-SSCP technique was applied to group feather white feather chickens according to different MHC B-LβII genotype,the different MHC B-LβII genotypes were collected from white feather chickens and peripheral blood lymphocytes were isolated.cCHMPS of the final concentrations100μg/mL,75μg/mL,50μg/mL,0μg/mL was added and cultured together for24h,and then the expression of lymphocyte IL-2,IL-12,IL-4mRNA was detected by using Real-time PCR method.【Result】(1)Traditional Chinese medicinal polysaccharide extraction of different doses could improve IL-2,IL-12,IL-4mRNA expression levels of chicken lymphocyte compared with the control group in each MHC B LβII genotype chickens(P<0.05).(2)And when the traditional Chinese medicines concentration was50μ-g/mL,the IL-2,IL-12,IL-4mRNA expression levels of AA and BC genotype chicken lymphocyte were higher than those of the same genotype.When the traditional Chinese medicines concentration was100μg/mL,the IL-2,IL-12,IL-4mRNA expression levels of BB genotype chicken lymphocyte were higher than those of the same genotype.【Conclusion】The traditional Chinese medicinal polysaccharide extraction could stimulate IL-2,IL-12,IL-4mRNA expression in different MHC B-LβII genotype chickens,and the optimum immunomodulatory doses vary in each MHC B-LβII genotype chickens.

关 键 词：中药复方多糖 MHCB-LβII基因 鸡淋巴细胞 荧光定量PCR 细胞因子 

分 类 号：S852[农业科学—基础兽医学]