中药复方多糖对不同MHC B-LβⅡ基因型蛋鸡禽流感抗体效价的影响  

Effects of Chinese Herbal Compound Polysaccharides on Antibody Potency of Avian Influenza in Different MHC B-LβⅡ Genotypes of Laying Hens

作　　者：刘晓婷[1] 朱晓庆[1] 马昭[1] 乔彦杰 张保军 谷新利[1] 商云霞[1] LIU Xiao-ting;ZHU Xiao-qing;MA Zhao;QIAO Yan-jie;ZHANG Bao-jun;GU Xin-li;SHANG Yun-xia(College of Animal Science and Technology, Shihezi University, Shihezi Xinjiang 832003, China)

机构地区：[1]石河子大学动物科技学院,新疆石河子832003

出　　处：《新疆农业科学》2017年第12期2329-2336,共8页Xinjiang Agricultural Sciences

基　　金：国家自然科学基金项目“一定纯度中药复方多糖对不同MHC B-LβⅡ基因型鸡免疫调节作用及其作用机制研究”(31460673)~~

摘　　要：【目的】研究肌注中药复方多糖对不同MHC B-LβⅡ基因型蛋鸡血清中禽流感抗体效价的影响。【方法】试验采用PCR-SSCP方法将500羽京红1号蛋鸡按不同基因型分组,再将各基因型组分成高、中、低剂量中药复方多糖组和空白对照组。各组试验鸡分别于8日龄肌注50、25和12.5 mg/mL中药复方多糖和生理盐水,0.2 mL/只,连续7 d,于27、35、42、49日龄从各组随机抽取5只试验鸡,翅下静脉采血并分离血清,用微量血凝抑制试验(HI)检测血清中H5与H9亚型禽流感抗体效价。【结果】免疫前,各剂量中药复方多糖组H5N1 Re-6和H9N2母源抗体平均效价高于对照组,且高剂量AA和BC基因型鸡血清中H5N1母源抗体效价显著高于对照组(P<0.05);免疫后7 d,AA基因型鸡高、中、低剂量组的H5亚型禽流感抗体效价均显著高于对照组(P<0.05),BC基因型鸡高剂量组H5亚型禽流感抗体效价显著高于对照组(P<0.05),BC基因型鸡高、中、低剂量组H9亚型禽流感抗体效价显著高于对照组(P<0.05);免疫后14 d,AA基因型鸡高、中剂量组H5亚型禽流感抗体效价显著高于对照组(P<0.05),BB基因型鸡中剂量组显著高于对照组(P<0.05),BC基因型鸡中剂量组H9亚型禽流感显著高于对照组(P<0.05);免疫后21 d,BC基因型鸡高、中剂量组H5亚型禽流感显著高于对照组(P<0.05),BC基因型鸡高剂量组显著高于对照组(P<0.05)。【结论】不同剂量中药复方多糖均能不同程度的提高各基因型蛋鸡血清中H5和H9亚型禽流感抗体效价,且以中剂量和高剂量效果好。【Objective】This experiment was conducted to study the effects of intramuscular injection of compound polysaccharides on avian influenza antibody titers in serum of different MHC B-LβII genotype laying hens.【Method】500chickens were divided into three groups according to different genotypes by PCR-SSCP method.The genotypes were divided into high,medium and low dose Chinese herbal compound polysaccharide groups and blank control group.The test chickens of each group were intramuscularly injected with50mg/mL,25mg/mL,12.5mg/mL Chinese herbal compound polysaccharide and saline,0.2mL/day for7days.At the age of27,35,42and49days,5chickens were randomly selected from each group and vein blood was collected from the wings for separation of serum.The titer of H5and H9subtype avian influenza antibodies in serum was measured by microhialysis inhibition test(HI).【Result】The results showed that,before immunization,the average titer of H5N1Re-6and H9N2maternal antibody was higher than that of the control group,and the titer of H5N1maternal antibody in AA and BC genotype of high-dose Chinese herbal compound polysaccharide group was significantly higher than that of control group(P<0.05).On the7day after immunization,the titer of H5subtype avian influenza antibody in AA genotype chickens in high,medium and low dose groups was significantly higher than that in control group(P<0.05),the titer of H5subtype avian influenza antibody in BC genotype high dose group was significantly higher than that in control group(P<0.05)and the titer of H9subtype avian influenza antibody in BC high,medium and low dose groups was significantly higher than that in control group(P<0.05);On the14days after immunization,the titer of H5subtype avian influenza antibody of AA genotype high and medium dose group was significantly higher than that of control group(P<0.05),the BB genotype in the middle dose group was significantly higher than that in the control group(P<0.05)and the H9genotype BC avian influenza in the middle dose group was significa

关 键 词：中药复方多糖 蛋鸡 MHCB-LβII基因 禽流感抗体效价 

分 类 号：S852[农业科学—基础兽医学]