三七护肝胶囊对小鼠酒精性肝损伤的保护作用  

Study on protective effect of Sanqi Hugan Capsules against alcoholic liver injury in mice

作　　者：程云飞[1] 吕晓君 

机构地区：[1]湖北省仙桃市第一人民医院,湖北仙桃433000 [2]湖北省药品监督检验研究院,湖北武汉430075

出　　处：《中国现代医学杂志》2018年第8期13-17,共5页China Journal of Modern Medicine

摘　　要：目的探讨三七护肝胶囊对50%乙醇所致小鼠酒精性肝损伤的保护作用。方法 SPF级昆明种雄性小鼠按体重随机分为空白对照组、模型对照组,以及三七护肝胶囊高、中、低剂量组,每组10只。连续灌胃30 d后,除空白对照组外,其余4组灌胃50%乙醇复制小鼠急性酒精性肝损伤模型。模型复制后16 h检测小鼠血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)含量,以及肝匀浆中丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、三酰甘油(TG)含量,并观察肝脏病理组织形态学变化。结果三七护肝胶囊高、中剂量组可降低小鼠血清ALT、AST和肝匀浆中MDA、TG含量,三七护肝胶囊高剂量组可提高肝匀浆中SOD、GSH活性。肝脏病理组织形态学结果显示,三七护肝胶囊高剂量组小鼠肝细胞脂肪变性程度较轻。结论三七护肝胶囊对酒精性肝损伤具有保护作用。Objective To study the protective effect of Sanqi Hugan Capsules(SHC)against alcoholic liver injury in mice induced by50%ethanol.Methods SPF-class Kunming male mice were randomly divided into5groups,i.e.normal control group,model control group,high-,medium-and low-dosage SHC groups with10in each group.After30days of continuous gavage,injuried liver models were made with50%ethanol orally except the normal control group.After16h,the serum content of ALT and AST and the levels of MDA,SOD,GSH and TG in liver homogenate were determined,the histopathologic changes of the hepatic tissues were observed.Results The serum concentrations of ALT and AST,and the liver homogenate levels of MDA and TG in the middle-dosage and high-dosage groups were significantly decreased,and the SOD and GSH levels in the liver homogenate of the highdosage group were significantly increased.Furthermore histopathological examination revealed fatty degeneration of the hepatic cells was significantly decreased in the high-dosage group.Conclusions SHC could protect liver against alcoholic injury.

关 键 词：三七护肝胶囊 酒精性肝损伤 小鼠 

分 类 号：R285.5[医药卫生—中药学]