虫草素对糖毒性胰腺INS-1E细胞保护作用  

Protection of Cordycepin in pancreatic INS-1E cells against glucotoxicity

作　　者：赵海燕[1] 陈立波[1] 黎丽萍[1] 黄兴 李静[1] 胡园园 吕术昕[1] ZHAO Haiyan;CHEN Libo;LI Liping;HUANG Xing;LI Jing;HU Yuanyuan;LYU Shuxin(Shenzhen Nanshan District People’s Hospital,Shenzhen 518052,China)

机构地区：[1]深圳市南山区人民医院,深圳518052

出　　处：《天津中医药》2018年第2期139-142,共4页Tianjin Journal of Traditional Chinese Medicine

基　　金：深圳市南山区科研基金项目(2016014);广东省中医药强省科研基金项目(20141241)

摘　　要：[目的]研究虫草素对糖毒性胰腺INS-1E细胞保护作用。[方法]建立胰腺INS-1E细胞糖毒性模型,给予虫草素浓度分别为6.125、12.5、25μmol/L处理,用CCK-8细胞技术试剂盒检测虫草素保护糖毒性胰腺INS-1E细胞存活率,用胰岛素酶联免疫吸附法(ELISA)试剂盒检测虫草素促进INS-1E细胞胰岛素分泌和合成作用,用蛋白免疫印迹(Western-blot)方法检测虫草素促进INS-1E细胞Akt磷酸化作用。[结果]虫草素能够提高糖毒性胰腺INS-1E细胞存活率,促进胰岛素的分泌和合成,提高蛋白激酶Akt的磷酸化作用。[结论]虫草素能对糖毒性胰腺INS-1E细胞具有一定的保护作用。[Objective]To study the role of Cordycepin in its protection of pancreatic INS-1E cells against glucotoxicity.[Methods]To establish glucotoxicity INS-1Ecell model,the INS-1E cells viability was observed under the action of different concentrations of Cordycepin(6.125,12.5,25μmol/L)by CCK-8,insulin secretion and synthesis was measured by ELISA Kit,phosphorylation of Akt was detected by Western-blot.[Results]Cordycepin improved viability in glucotoxicity pancreatic INS-1E cells,promoted insulin secretion and synthesis,increased phosphorylation of protein kinase Akt.[Conclusion]Cordycepin have protective effect in glucotoxicity pancreatic INS-1E cells.

关 键 词：糖毒性 虫草素 AKT 胰腺 2NS-1E 

分 类 号：R285.5[医药卫生—中药学]