一贯煎对子痫前期大鼠胎盘NF-κB mRNA与MMP-9 mRNA表达影响  

Effect of Yiguanjian on expression of NF-κB m RNA and MMP-9 mRNA in placenta of preeclampsia model rat

作　　者：黎燕 曹保利[2] 刘筠[2] LI Yan;CAO Baoli;LIU Yun(Tianjin University of Traditional Chinese Medicine, Tianjin 300193,China;Department of Obstetrics and Gynecology, Nankai Hospital of Tianjin,Tianjin 300100, China)

机构地区：[1]天津中医药大学,天津300193 [2]天津市南开医院妇产科,天津300100

出　　处：《天津中医药》2018年第3期214-216,共3页Tianjin Journal of Traditional Chinese Medicine

基　　金：天津市卫生和计划生育委员会中医中西结合科研课题项目(2015032)

摘　　要：[目的]探讨中药一贯煎对子痫前期大鼠胎盘核转录因子-κB mRNA(NF-κB mRNA)和基质金属蛋白酶-9mRNA(MMP-9 mRNA)表达的影响。[方法]建立子痫前期Wistar大鼠模型,并灌胃给予中药一贯煎,监测给药前后大鼠血压及24 h尿蛋白变化。PCR法检测孕鼠胎盘NF-κB m RNA及MMP-9 m RNA的表达。[结果]1)NF-κB mRNA:与正常妊娠组相比,模型对照组及一贯煎治疗组均升高,与模型对照组相比,一贯煎治疗组降低,且差异均有显著性(P<0.05)。2)MMP-9 m RNA:与正常妊娠组相比,模型对照组与一贯煎治疗组均降低,与模型对照组相比,一贯煎治疗组有所升高,且差异均有显著性(P<0.05)。[结论]一贯煎通过降低子痫前期大鼠胎盘NF-κB mRNA的表达,升高MMP-9mRNA的表达治疗子痫前期大鼠。[Objective]To explore of the effect of Yiguanjian on expression of NF-κB mRNA and MMP-9mRNA in placenta of preeclampsia model rat.[Methods]The preeclampsia rat model was established and intragastric administration of traditional Chinese medicine Yiguanjian.The changes of blood pressure and24h urine protein of rats were monitored before and after dosing.The PCR method to measure the expression of NF-κB mRNA and MMP-9mRNA in placenta of pregnant rats.[Results]1)The expression of NF-κB mRNA in model control group and Yiguanjian treat group was higher compared with the normal group,with statistical significance(P<0.05),and the expression in Yiguanjian treat group was lower compared with model contonl group,with statistical significance(P<0.05).2)The expression of MMP-9mRNA in model control group and Yiguanjian treat group was lower compared with the normal group,with statistical significance(P<0.05),and the expression in Yiguanjian treat group was higher compared with model control group,with statistical significance(P<0.05).[Conclusion]Yiguanjian treatments preeclampsia model rat by reducing the expression of NF-k-B mRNA and rising the expression of MMP-9mRNA in placenta.

关 键 词：一贯煎 子痫前期 NF-κBmRNA MMP-9MRNA 

分 类 号：R285.5[医药卫生—中药学]