足月临产子宫平滑肌组织PPARα表达水平与子宫收缩性和炎性因子的相关性  

The correlation between expression level of PPARα and uterine contraction, inflammatory factor level in term-labor uterine smooth muscle

作　　者：高莉 李长忠[2] GAO Li;LI Chang-zhong(Department of Obstetrics and Gynaecology, the No.95 Hospital of PLA, Fujian, Putian 351100, China;Department of Gynaecology, Shandong Provincial Hospital, Jinan 250021, China)

机构地区：[1]解放军第九五医院产科,福建莆田351100 [2]山东省立医院妇科,济南250021

出　　处：《中国医学前沿杂志（电子版）》2017年第12期49-52,共4页Chinese Journal of the Frontiers of Medical Science(Electronic Version)

基　　金：山东省自然科学基金资助项目(ZR2013HM060)

摘　　要：目的研究足月临产子宫平滑肌组织过氧化物酶体增殖物激活受体α(peroxisome proliferator activated receptorsα,PPARα)表达水平与子宫收缩性和炎性因子水平的相关性。方法收集2012年5月至2016年8月解放军第九五医院剖宫产产妇的子宫平滑肌标本,根据是否临产,将子宫平滑肌标本分为足月未临产(term non-labor,TNL)组和足月临产(term labor,TL)组。采用张力换能器检测两组标本肌肉收缩力,采用酶联免疫吸附测定检测两组标本PPARα和炎性因子表达水平。结果 TL组子宫平滑肌肌条收缩的振幅显著大于TNL组(P<0.05),收缩循环时间显著短于TNL组(P<0.05);TL组子宫平滑肌组织中PPARα表达水平显著低于TNL组(P<0.05),核因子-κB(NF-κB)、肿瘤坏死因子-α(TNF-α)、白介素(IL)-1β、IL-6水平均显著高于TNL组(P<0.05);PPARα表达水平与子宫平滑肌肌条收缩的振幅及NF-κB、TNF-α、IL-1β、IL-6水平均呈负相关(r分别为:-0.642、-0.692、-0.592、-0.553、-0.609;P<0.05),与子宫平滑肌肌条收缩的循环时间呈正相关(r=0.628)。结论足月临产子宫平滑肌组织低水平PPARα能够增强子宫肌收缩力,增加炎性因子浸润,参与分娩启动。Objective To investigate the correlation between the expression level of peroxisome proliferator activated receptorα(PPARα)and uterine contraction,inflammatory factor level in term-labor uterine smooth muscle.Method Uterine smooth muscle specimens of puerperant who used uterine-incision delivery in the No.95Hospital of PLA from May2012to August2016were collected.According to labor or not,uterine smooth muscle specimens were grouped into full term non-labor(TNL)group and term labor(TL)group.The contractile force of uterine smooth muscle of two groups was measured by tension transducer,and the expression levels of PPARαand inflammatory factors of the two groups were measured by ELISA.Result Uterine smooth muscle contraction amplitude of TL group was significantly higher than that of TNL group(P<0.05),contraction cycle time was significantly shorter than that of TNL group(P<0.05);PPARαexpression level in uterine smooth muscle of TL group was significantly lower than that of TNL group(P<0.05),NF-κB,TNF-α,IL-1β,IL-6levels were significantly higher than those of TNL group(P<0.05);PPARαexpression level was negatively correlated to uterine muscle contraction amplitude and NF-κB,TNF-α,IL-1β,IL-6level(r=-0.642,-0.692,-0.592,-0.553,-0.609;P<0.05),positively correlated to uterine smooth muscle contraction cycle(r=0.628).Conclusion Low-expression of PPARαin term-labor uterinesmooth muscle can enhance uterine muscle contraction,increase infiltration of inflammatory cytokines,and then involve in the initiation of parturition.

关 键 词：早产 过氧化物酶体增殖物激活受体Α 炎性因子 

分 类 号：R714[医药卫生—妇产科学]