慢性应激抑郁型黄褐斑小鼠模型的建立  

Establishment of Mouse Model of Chronic Stress Depression Chloasma

作　　者：申宇鸿[1] 赵广[2] 耿龙[3] 孟如松[2] 李菲[2] 郭广进[2] SHEN Yu-hong;ZHAO Guang;GENG Long;MENG Ru-song;LI Fei;GUO Guang-jin(Department of Dermatology,Beijing Hepingli Hospital,Beijing 100013,China;Department of Dermatology, Air Force General Hospital of PLA,Beijing 100142,China;Department of Dermatology,the First Hospital of China Medical University,Shenyang 110122,Liaoning,China)

机构地区：[1]北京市和平里医院皮肤科,北京100013 [2]解放军空军总医院皮肤病医院皮肤科,北京100142 [3]中国医科大学附属第一医院皮肤科,辽宁沈阳110122

出　　处：《中国美容医学》2018年第2期94-97,共4页Chinese Journal of Aesthetic Medicine

摘　　要：目的:根据黄褐斑主要发病因素,综合作用于小鼠建立抑郁型黄褐斑小鼠模型,并与现有方法建立的模型进行比较。方法:取小鼠30只,随机分为3组:正常组6只、紫外线组12只、紫外线+黄体酮+抑郁组12只。造模时间第一批21d,第二批28d,然后对各组小鼠皮肤和肝脏SOD、MDA含量,皮肤黑色素细胞个数、数密度和平均光密度值进行统计分析。结果:黄体酮+紫外线+慢性应激抑郁模型建立法造模较其他方法更能导致皮肤丙二醛(Malondialdehyde,MDA)升高和超氧化物歧化酶(Superoxide Dismutase,SOD)降低以及皮肤黑色素细胞的增加,差异均有统计学意义(P<0.01)。且同时停止紫外线照射后1周,黄体酮+紫外线+抑郁组色素沉着消退慢,黑色素数量仍高于其他模型组。结论:黄体酮+紫外线+慢性应激抑郁模型建立的黄褐斑小鼠模型获得成功。Objective To establish and validate mouse model of depressive chloasma according to the main pathogenic factors of melasma,and compared with other methods.Methods30mice were randomly divided into3groups:6mice in normal group,12mice in ultraviolet group,12mice in ultraviolet+progesterone+depression group.The first batch of21days,the second batch of28days,then the skin and liver SOD,MDA contents,the numbers of melanocytes,the numbers density and the average optical density values of each group of mice were statistically analyzed.Results Progesterone and UV+chronic stress depression model modeling method is superior to other methods,it can more effectively lead to skin malondialdehyde(MDA)increased and the activity of superoxide dismutase(SOD)decreased and skin melanocytes increased(P<0.01).After a week and stop after ultraviolet irradiation and UV+progestin depression group pigmentation faded slowly,the number of melanin was still higher than the model group.Conclusion Mouse model of chloasma established by progesterone+ultraviolet+chronic stress depression is successful.

关 键 词：黄褐斑 抑郁型 小鼠模型 丙二醛 超氧化物歧化酶 

分 类 号：R758.42[医药卫生—皮肤病学与性病学]