大豆酶解蛋白对凡纳滨对虾幼虾生长性能、血清生化指标、非特异性免疫力和抗病力的影响  

Effects of Enzymolytic Soybean Meal on Growth Performance, Serum Biochemical Indices, Non-specific Immunity and Disease Resistance of Juvenile Litopenaeus vannamei

作　　者：王铵静 杨奇慧[1] 谭北平[1] 肖伟伟 贾静 董晓慧[1] 迟淑艳[1] 刘泓宇[1] 章双[1] WANG An-jing;YANG Qi-hui;TAN Bei-ping;XIAO Wei-wei;JIA Jing;DONG Xiao-hui;CHI Shu-yan;LIU Hong-yu;ZHANG Shuang(Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China;Chengdu Mytech Biotech Co., Ltd, Chengdu 610222,China)

机构地区：[1]广东海洋大学水产学院,广东湛江524088 [2]成都美溢德生物技术有限公司,四川成都610222

出　　处：《广东海洋大学学报》2018年第1期14-21,共8页Journal of Guangdong Ocean University

基　　金：农业部公益性行业科研专项(201003020);广东省产业技术与开发专项资金项目(2013B021100017);广东省自然科学基金(2016A030313749);成都美溢德生物技术有限公司项目(B16017)

摘　　要：【目的】研究大豆酶解蛋白对幼虾生长性能、血清生化指标、非特异性免疫力和抗病力的影响。【方法】凡纳滨对虾(Litopenaeus vannamei)基础饲料添加质量分数0(对照)、1.0%、1.5%、2.0%、2.5%、3.0%、3.5%和4.0%大豆酶解蛋白,配制8种等氮等脂饲料,饲喂凡纳滨对虾幼虾56 d。【结果】1.5%组的增重率和特定生长率最高,显著高于2.0%—4.0%添加组(P<0.05);1.0%和1.5%组全虾粗蛋白质含量高于对照组(P<0.05),对照、1.0%和1.5%组蛋白质沉积率高于其他各组(P<0.05);饲料中添加1.5%~4.0%大豆酶解蛋白可显著提高肌肉磷含量(P<0.05),3.0%组最大;1.0%、1.5%和2.0%组血清胆固醇含量低于对照组(P<0.05);饲料中添加1.5%~4.0%大豆酶解蛋白可显著提高血清中溶菌酶活性(P<0.05),添加1.0%~3.0%大豆酶解蛋白可显著提高血清中酚氧化物酶活性(P<0.05),2.5%和3.0%组血清中超氧化物歧化酶活性与1.0%和1.5%组血清中酸性磷酸酶活性高于对照组(P<0.05),添加大豆酶解蛋白可显著提高血清中碱性磷酸酶活性(P<0.05),2.5%组最大;哈维弧菌(Vibrio harveyi)攻毒7 d后,1.0%组累积死亡率最低。饲料中添加大豆酶解蛋白未提高凡纳滨对虾的生长性能,添加剂量高于3.5%可导致生长性能下降,添加质量分数1.0%大豆酶解蛋白可明显提高凡纳滨对虾的抗病力。【结论】饲料中添加大豆酶解蛋白不能提高凡纳滨对虾的生长性能,高于3.5%添加组的生长性能下降;添加质量分数1.0%大豆酶解蛋白可明显提高凡纳滨对虾的抗病力。【Objectives】This study is to investigate the effects of enzymolytic soybean meal on the growth performance,serum biochemical indices,non-specific immunity and disease resistance of juvenile Litopenaeus vannamei.【Method】Eight isonitrogenous and isolipid diets were prepared by adding enzymolytic soybean meal to the final of0,1.0%,1.5%,2.0%,2.5%,3.0%,3.5%and4.0%groups of the basal diet,and fed to juvenile Litopenaeus vannamei for56d.【Result】The maximum weight gain rate(WGR)and specific growth rate(SGR)occurred in the1.0%enzymolytic soybean meal groups,and was significantly higher(P<0.05)than those of the2.0%to4.0%groups.The body crude protein content in the1.0%and1.5%groups were significantly higher than the control group(P<0.05).Protein deposition rate(PDR)in control,1.0%and1.5%group were significantly higher than that in other groups(P<0.05).Muscle phosphorus content was significantly increased by dietary supplying1.5%to4.0%enzymolytic soybean meal(P<0.05),and a highest value was found in3.0%group.Serum cholesterol(CHOL)in1.0%,1.5%and2.0%groups were significantly lower than that in the control(P<0.05).The activity of serum lysozyme(LSZ)was significantly increased by dietary supplyment of1.5%to4.0%enzymolytic soybean meal(P<0.05).The activity of serum phenoloxidase(PO)was significantly increased by dietary supplyment of1.0%to3.0%enzymolytic soybean meal(P<0.05).The activity of serum superoxide dismutase(SOD)in1.5%and2.0%group and the activity of serum acid phosphatase(ACP)in1.0%and1.5%groups were significantly higher than those in the control(P<0.05).Serum alkaline(AKP)activity was significantly increased in diets supplement with enzymolytic soybean meal(P<0.05),and the highest value occurred in the2.5%group.Shrimp were challenged by Vibrio harveyi for the next7days.The minimum cumulative mortality rate was occurred in1.0%group.Diets with>3.5%enzymolytic soybean meal can inhibit the growth performance of juvenile and supplement of1.0%enzymolytic soybean meal can obviously improve the disease resistance

关 键 词：大豆酶解蛋白 凡纳滨对虾 幼虾 生长性能 血清生化指标 非特异性免疫力 抗病力 

分 类 号：S963.73[农业科学—水产养殖]