石墨烯量子点荧光猝灭-恢复法测定Cu^(2+)与谷胱甘肽的含量  

Detection of copper ions and glutathione based on off-on fluorescent graphene quantum dots

作　　者：宋凤娟 艾永玲 钟文英[1,2] 王菁[1,2] SONG Fengjuan;AI Yongling;ZHONG Wenying;WANG Jing(Key Laboratory of Biomedical Functional Materials,Nanjing 210009,China;Key Laboratory of Drug Quality Control and Pharmacovigilance,Ministry of Education ,China Pharmaceutical University,Nanjing 210009,China)

机构地区：[1]中国药科大学医药生物功能材料重点实验室,南京210009 [2]中国药科大学药物质量与安全预警教育部重点实验室,南京210009

出　　处：《中国药科大学学报》2018年第1期87-92,共6页Journal of China Pharmaceutical University

基　　金：国家自然科学基金资助项目(No.21405178);江苏省自然科学基金资助项目(No.BK20161455);中央高校基本科研业务费专项基金资助项目(No.2016ZZD004);中国药科大学医药生物功能材料重点实验室开放基金资助项目~~

摘　　要：通过在温和条件下氧化石墨粉的方法合成高分散性、高荧光强度的石墨烯量子点(GQDs),并运用此GQDs对Cu^(2+)和GSH具有荧光猝灭-恢复的效应,建立对这两种物质简便、快捷的检测方法。Cu^(2+)与GSH的浓度分别在1.0~10.0 mmol/L、0.1~1.0 mmol/L范围内与GQDs的荧光强度呈良好线性关系,检测限分别为0.01和0.1 mmol/L。此外,在实际样品的检测中,加标法测得的回收率分别为93%~101%、96%~107%。该方法操作简便,测定准确,精密度高,且常见的金属离子及潜在共存物质对Cu^(2+)与GSH的检测均没有干扰。We developed a rapid method to detect Cu2+and glutathione(GSH)based on the fluorescence quenching-recovery properties of graphene quantum dots(GQDs),which were synthesized by the gentle oxidation of graphite powder.The results revealed that the fluorescence intensity of GQDs versus concentration of Cu2+and GSH had good linearity,with the detection limits of0.01and0.1mmol/L,respectively.The fluorescence quenching was linearly proportional to the concentrations of Cu2+ranging from1.0to10.0mmol/L;the fluorescence intensity was linearly enhanced with the concentrations of GSH ranging from0.1to1.0mmol/L.In addition,the method was successfully applied to the determination of real samples with recoveries falling between93%-101%and96%-107%,respectively.This method is simple,accurate and precise.There was no interference with other familiar co-existing metal ions and potential materials.

关 键 词：石墨烯量子点 CU2+ 谷胱甘肽 荧光猝灭-恢复 含量测定 

分 类 号：R971[医药卫生—药品]