电针对吗啡成瘾大鼠中枢多巴胺受体的影响  

Effect of Electroacupuncture on Central Dopamine Receptor in Morphine-dependent Rats

作　　者：梁艳[1] 曾亮[1] 高晓悦 张晴 来祯 LIANG Yan;ZENG Liang;GAO Xiao-yue;ZHANG Qing;LAI Zhen(Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China)

机构地区：[1]上海中医药大学附属岳阳中西医结合医院,上海200437

出　　处：《上海针灸杂志》2018年第3期324-329,共6页Shanghai Journal of Acupuncture and Moxibustion

基　　金：国家自然科学基金青年基金项目(81403473);上海市卫生和计划生育委员会科研课题资助项目(20134292)

摘　　要：目的观察吗啡成瘾大鼠中脑腹侧被盖区(VTA)、伏隔核(NAc)、前额皮层(PFC)内多巴胺(DA)受体变化及电针的调节作用。方法 30只雄性Wistar大鼠随机分为对照组、模型组和电针组,每组10只。采用吗啡自身给药成瘾大鼠模型,电针组选取双侧T5、L2夹脊穴,电针连续治疗4 d。Western Blotting法观察各组大鼠脑VTA、NAc、PFC内D1、D2受体含量的变化。结果治疗后,相对于模型组,电针组大鼠的吗啡摄入量明显减少(P<0.05)。模型组大鼠脑VTA内多巴胺D2受体减少(P<0.05);NAc内多巴胺D1受体增加(P<0.05),D2受体减少(P<0.05);PFC内多巴胺D1受体减少(P<0.05),D2受体增加(P<0.05)。电针治疗能调节上述脑区发生变化的D1、D2受体含量,使之趋于正常。结论电针治疗可以在一定程度上抑制成瘾大鼠对吗啡的渴求;吗啡成瘾后多巴胺投射通路中的多巴胺受体发生了一定程度的适应性改变,电针治疗可以调节DA受体的异常表达,对吗啡成瘾大鼠的多巴胺受体起到保护作用。Objective To observe the change of dopamine receptor in ventral tegmental area(VTA),nucleus accumbens(NAc)and prefrontal cortex(PFC)in morphine-dependent rats,and the regulation effect of electroacupuncture(EA).Method Thirty male SD rats were randomized into a control group,a model group and an EA group,10rats each.Morphine-dependent rat models were induced by morphine self-administration.In the EA group,bilateral Jiaji points(EX-B2)of T5and L2were selected,and the EA intervention lasted4d.Western blotting method was adopted to observe the change of the contents of dopamine receptor D1and D2in VTA,NAc and PFC of the rats.Result After the intervention,compared to the model group,the morphine intake was reduced significantly in the EA group(P<0.05);the level of D2declined significantly in VTA of rats in the model group(P<0.05);the level of D1increased significantly and D2declined significantly in NAc of rats in the model group(P<0.05);the level of D1declined significantly and D2increased significantly in PFC of rats in the model group(P<0.05);EA produced regulation effect on the altered contents of D1and D2in the cerebral areas mentioned above,approaching the normal level.Conclusion EA can inhibit the hunger of addiction rats for morphine to some extent;the contents of dopamine receptors in dopamine projection pathway will take adaptive changes after morphine addiction,while EA can regulate the abnormal expressions of dopamine receptors,producing a protective effect on dopamine receptors of morphinedependent rats.

关 键 词：电针 吗啡依赖 行为 成瘾 多巴胺受体 

分 类 号：R2-03[医药卫生—中医学]