电针对2型糖尿病模型大鼠下丘脑IRS-1的影响  

Effect of Electroacupuncture on Hypothalamic IRS-1 in a Rat Model of T2DM

作　　者：蔡少康 李颖波[1] 杨丰 樊永智[1] 董军立[1] 吴群[1] 蔡毅[1] CAI Shao-kang;LI Ying-bo;YANG Feng;FAN Yong-zhi;DONG Jun-li;WU Qun;CAI Yi(Wuhan Central Hospital,Wuhan 430000,China;Hubei Yunmeng County People’s Hospital,Xiaogan 432000,China)

机构地区：[1]武汉市中心医院,武汉430000 [2]湖北省云梦县人民医院,孝感432000

出　　处：《上海针灸杂志》2018年第3期330-334,共5页Shanghai Journal of Acupuncture and Moxibustion

基　　金：湖北省武汉市重点项目(WX17B02)

摘　　要：目的观察电针对2型糖尿病(T2DM)模型大鼠下丘脑中胰岛素受体底物蛋白质-1(IRS-1)的影响。方法将60只Wistar大鼠随机分为正常组15只和观察组45只。观察组采用高能饲料诱导造模,8星期造模成功后将观察组随机分为造模组、治疗组和阻滞组,每组15只。治疗组给予电针治疗;阻滞组给予电针配合脑室灌注磷脂酰肌醇-3羟基激酶(PI3K)阻滞剂治疗。治疗8星期后,采用血糖仪检测各组大鼠空腹血糖(FPG),采用ELISA法检测空腹血清胰岛素(Fins),计算胰岛素抵抗指数(IAI),并采用免疫组化SABC法检测大鼠IRS-1含量的表达。结果与正常组比较,造模组的FPG、Fins显著上升(均P<0.01),IAI、IRS-1表达显著降低(P<0.01)。与造模组比较,治疗组的FPG、Fins显著下降(均P<0.01),IAI、IRS-1表达显著上升(P<0.01)。与阻滞组比较,治疗组的FPG、Fins均显著下降(P<0.05,P<0.01),IAI、IRS-1表达显著上升(P<0.01)。结论电针可通过调节下丘脑IRS-1的表达,改善T2DM大鼠的FPG、Fins及胰岛素敏感性。Objective To investigate the effect of electroacupuncture on hypothalamic insulin receptor substrate1(IRS-1)in a rat model of type2diabetes mellitus(T2DM).Methods Sixty Wistar rats were randomized to a normal group(15rats)and an observation group(45rats).In the observation group,a rat model of T2DM was made by high-energy diet induction.After the model was successfully made8weeks later,the observation group was randomized to model making,treatment and blocker groups,15rats each.The treatment group received electroacupuncture and the blocker group,electroacupuncture plus intraventricular perfusion of phosphatidylinositol3-hydroxyl kinase(PI3K)blocker.After8weeks of treatment,fasting plasma glucose(FPG)was measured using a glucometer,fasting insulin(Fins)was determined by ELISA,insulin resistance index(IRI)was calculated and IRS-1expression was examined by SABC immunohistochemistry assay in every group of rats.Results FPG and Fins increased significantly(both P<0.01)and IRI and IRS-1expression decreased significantly(P<0.01)in the model making group compared with the normal group.FPG and Fins decreased significantly(both P<0.01)and IRI and IRS-1expression increased significantly(P<0.01)in the treatment group compared with the model making group.FPG and Fins decreased significantly(P<0.05,P<0.01)and IRI and IRS-1expression increased significantly(P<0.01)in the treatment group compared with the blocker group.Conclusion Electroacupuncture can improve FPG,Fins and insulin sensitivity by regulating hypothalamic IRS-1expression in T2DM rats.

关 键 词：针刺疗法 糖尿病 2型 胰岛素受体底物蛋白 血糖 血清胰岛素 胰岛素抵抗指数 大鼠 下丘脑 

分 类 号：R2-03[医药卫生—中医学]