川芎中总生物碱、总酚酸和总挥发油对大鼠离体子宫平滑肌收缩活动的抑制作用  

Inhibition Effect of Total Alkaloid,Total Phenolic Acid and Total Volatile Oil from Ligusticum chuanxiong on the Contraction of Isolated Uterine Smooth Muscle of Rats

作　　者：刘娟[1,2] 蒲忠慧 彭成[1,2] 张鑫[1,2] 李文兵 戴鸥[1,2] LIU Juan;PU Zhonghui;PENG Cheng;ZHANG Xin;LI Wenbing;DAI Ou(College of Pharmacy,Chengdu University of TCM/Key Lab of Standardization of Chinese Herbal Medicine,Ministry of Education/State Key Lab Breeding Base of Systematic R&D and Utilization of Chinese Medicine Resource,Chengdu 611137,China;Institute of Innovative Medicine Ingredients of Southwest Specialty Medicinal Materials,Chengdu University of TCM,Chengdu 611137,China;Sichuan Neo-Green Pharmaceutical Technology Development Co.,Ltd.,Sichuan Pengzhou 610031,China)

机构地区：[1]成都中医药大学药学院/教育部中药材标准化重点实验室/中药资源系统研究与开发利用省部共建国家重点实验室培育基地,成都611137 [2]成都中医药大学西南特色药材创新药物成分研究所,成都611137 [3]四川新绿色药业科技发展股份有限公司,四川彭州610031

出　　处：《中国药房》2018年第5期621-624,共4页China Pharmacy

基　　金：四川省科技支撑计划项目(No.2015SZ0031)

摘　　要：目的:考察川芎中总生物碱、总酚酸和总挥发油对大鼠离体子宫平滑肌收缩活动的抑制作用,为阐明川芎活血调经的传统功效提供依据。方法:取大鼠离体子宫平滑肌浸泡于洛氏液中,以二甲基亚砜为空白对照、盐酸维拉帕米为阳性对照,采用Power Lab生理记录仪记录子宫平滑肌收缩曲线,通过观察收缩活动力、收缩张力变化来评价川芎中总生物碱、总酚酸(质量浓度均分别为0.025、0.05、0.1 mg/m L)和总挥发油(0.04、0.08、0.16 mg/m L)对缩宫素致大鼠离体子宫平滑肌收缩的抑制作用,并计算子宫平滑肌收缩活动力抑制率和收缩张力抑制率。结果:加入缩宫素后大鼠离体子宫平滑肌的收缩活动力、收缩张力较加入缩宫素前均显著升高(P<0.05或P<0.01),不同质量浓度3类川芎组分均可显著降低子宫平滑肌的收缩活动力、收缩张力(P<0.05或P<0.01)。与空白对照比较,除0.025 mg/m L总酚酸外,给予其余浓度药物或组分后子宫平滑肌的收缩活动力抑制率和收缩张力抑制率均显著升高(P<0.05或P<0.01)。结论:川芎中总生物碱、总酚酸和总挥发油对缩宫素致大鼠离体子宫平滑肌收缩均有一定的抑制作用,这为川芎活血调经的用法提供了参考依据。OBJECTIVE:To investigate the inhibition effect of total alkaloid,total phenolic acid and total volatile oil from Ligusticum chuanxiong on the contraction of the isolated uterine smooth muscle of rats,and to provide reference for clarifying the promoting blood circulation to restore menstrual flow traditional efficacy of L.chuanxiong.METHODS:The isolated uterine smooth muscle of rats were collected and soaked in Locke’s solution.Using dimethyl sulfoxide as blank control,verapamil hydrochloride as positive control,the contraction curves of the isolated uterine smooth muscle in rats were recorded with PowerLab electrophysiolograph.Inhibitory effects of total alkaloid,total phenolic acid(mass concentrations of 0.025,0.05,0.1 mg/mL)and total volatile oil(0.04,0.08,0.16 mg/mL)on the contraction of the isolated uterine smooth muscle of rats induced by oxytocin were evaluated by observing the changes of contraction activity and contraction tension.Inhibitory rates of contraction activity and contraction tension of uterine smooth muscle were calculated.RESULTS:The contraction activity and contraction tension of uterine smooth muscle in rats were increased significantly after treated with oxytocin(P<0.05 or P<0.01).Different concentrations of 3 types of compounds from L.chuanxiong all decreased contraction activity and contraction tension of uterine smooth muscle significantly(P<0.05 or P<0.01).Compared with blank control,except for 0.025 mg/mL total phenolic acid,the inhibitory rates of contraction activity and contraction tension of uterine smooth muscle were all decreased significantly(P<0.05 or P<0.01)after treated with other concentration drugs or components.CONCLUSIONS:The total alkaloid,total phenolic acid and total volatile oil from L.chuanxiong show certain inhibitory effect on the contraction of isolated uterine smooth muscle in rats,which provide reference for promoting blood circulation to restore menstrual flow use of L.chuanxiong.

关 键 词：川芎 总生物碱 总酚酸 总挥发油 离体子宫 大鼠 平滑肌收缩 

分 类 号：R965.1[医药卫生—药理学]