干细胞对环孢素肾病大鼠肾组织转化生长因子β1的影响  

Effect of mesenchymal stem cells on transforming growth factor-β1 in renal tissue of chronic cyclosporine nephropathy in rats

作　　者：张勇[1] 张巍[2] 张剑平[2] 卓文利[2] 吴卫真[2] 谭建明[2] Zhang Yong;Zhang Wei;Zhang Jianping;Zhuo Wenli;Wu Weizhen;Tan Jianming(the Second Military Medical University,Shanghai 200433,China;Department of Urology,Fuzhou General Hospital of Nanjing Military Command,Fuzhou 350025,China)

机构地区：[1]第二军医大学,上海200433 [2]南京军区福州总医院泌尿外科,福州350025

出　　处：《中华细胞与干细胞杂志（电子版）》2017年第1期18-22,共5页Chinese Journal of Cell and Stem Cell（Electronic Edition）

基　　金：福建省重大专项课题(2012YZ0001)

摘　　要：目的观察干细胞治疗技术对环孢素肾病大鼠肾组织转化生长因子β1(TGF-β1)的影响。方法实验大鼠分成3组,每组10只大鼠:C组:正常对照组;H组:模型组;B组:骨髓间充质干细胞治疗组。28 d处死大鼠切取肾脏,免疫组织化学染色、RT-PCR、Western blot测定肾组织TGF-β1、α-平滑肌肌动蛋白(α-SMA)的表达水平。采用单因素方差分析比较各组数据。结果 (1)免疫组织化学染色结果显示:与C组比较,H组、B组TGF-β1(0.899±0.046vs 9.524±1.232,6.437±0.728;P<0.01)、α-SMA(2.427±0.188 vs 11.912±2.610,9.232±2.268;P<0.01)表达水平均显著升高,差异具有统计学意义。与H组比较,B组大鼠的TGF-β1(9.524±1.232 vs 6.437±0.728;P<0.01)、α-SMA(11.912±2.610 vs 9.232±2.268;P<0.01)表达水平显著下降,差异具有统计学意义;(2)RT-PCR结果显示:与C组比较,H组、B组TGF-β1(0.119±0.003 vs 0.826±0.004,0.651±0.004;P<0.01)、α-SMA(0.370±0.006 vs 0.900±0.007,0.642±0.007;P<0.01)m RNA水平均显著升高,差异具有统计学意义(P<0.05)。与H组比较,B组大鼠的TGF-β1(0.826±0.004 vs 0.651±0.004;P<0.01)、α-SMA(0.900±0.007 vs 0.642±0.007;P<0.01)m RNA水平显著下降,差异具有统计学意义;(3)Western blot结果显示:与C组比较,H组、B组TGF-β1(0.218±0.004 vs 0.825±0.003,0.650±0.006;P<0.01)、α-SMA(0.263±0.003vs 0.841±0.003,0.615±0.003;P<0.01)蛋白水平均显著升高,差异具有统计学意义。与H组比较,B组大鼠的TGF-β1(0.825±0.003 vs 0.650±0.006;P<0.01)、α-SMA(0.841±0.003 vs 0.615±0.003;P<0.01)蛋白水平显著下降,差异具有统计学意义。结论骨髓间充质干细胞治疗可以下调环孢素肾病大鼠肾组织TGF-β1表达水平,减少α-SMA表达水平,从而改善大鼠肾间质纤维化程度。Objectives To investigate the effect of mesenchymal stem cells on transforming growth factor-β1 in the renal tissue of rats with chronic cyclosporine nephropathy.Methods The experimental animals were divided into 3 groups:Group C:normal group(control);Group H:chronic cyclosporine nephropathy group;Group B:mesenchymal stem cells therapy group.On day 28 kidney tissue was evaluated for the expression levels of TGF-β1 andα-SMA by immunohistochemistry,RT-PCR,and Western blot.Results(1)Immunohistochemistry results:Compared with group C,TGF-β1(0.899±0.046 vs 9.524±1.232,6.437±0.728;P<0.01),α-SMA(0.370±0.006 vs 0.900±0.007,0.642±0.007;P<0.01)in model group and group B are significantly increased.Compared with group H,expression levels of the above proteins in the groups B decreased(TGF-β1:9.524±1.232 vs 6.437±0.728;P<0.01;α-SMA:11.912±2.6109.232±2.268,P<0.01).(2)RT-PCRresults:Compared with groupC,TGF-β1(0.119±0.003 vs0.826士0.004,0.651±0.004;P<0.01),α-SMA(0.370±0.006 vs 0.900±0.007,0.642±0.007;P<0.01)in group H and group B are significantly increased.Compared with group H,expression levels of the above proteins in groups B decreased(TGF-β1:0.826±0.004 vs 0.651±0.004;P<0.01;α-SMA:0.900±0.007 vs 0.642±0.007;P<0.01)(3)Western blot results:Compared with group C,TGF-β1(0.218±0.004 vs 0.825±0.003,0.650±0.006;P<0.01),α-SMA(0.370±0.006 vs 0.900±0.007,0.642±0.007;P<0.01)in model group and group B significantly increased.Compared with group H,expression levels of the above proteins decreased in group B(TGF-β1:0.825±0.003 vs 0.650±0.006;P<0.01;0.841±0.003 vs 0.615±0.003;P<0.01).Conclusion Bone mesenchymal stem cells can mitigate renal interstitial fibrosis by decreasing the expression of TGF-β1 andα-SMA.

关 键 词：亲环素类 肾病 骨髓 间质干细胞 转化生长因子Β1 

分 类 号：R692[医药卫生—泌尿科学]