苯并噁唑类化合物K310对LPS刺激RAW264.7巨噬细胞的抗炎作用  

The Anti-inflammatory Effect of the Benzoxazole Compound K310 on the LPS-stimulated RAW264.7 Macrophages

作　　者：赵波波[1] 莫春芬 郭慧杰 邹强[1,2] Zhao Bobo;Mo Chunfen;Guo Huijie;Zou Qiang(School of Basic Medical Sciences,Chengdu Medical College,Chengdu 610500,China;Center for Scientific Research,Chengdu Medical College,Chengdu 610500,China)

机构地区：[1]成都医学院基础医学院,成都610500 [2]成都医学院科研实验中心,成都610500

出　　处：《成都医学院学报》2018年第1期1-5,共5页Journal of Chengdu Medical College

基　　金：国家自然科学基金项目(No:81273530)

摘　　要：目的探索K310对脂多糖(LPS)诱导RAW264.7巨噬细胞的抗炎作用及机制。方法在LPS诱导RAW264.7巨噬细胞的模型基础上,采用CCK-8方法检测不同浓度K310对RAW264.7巨噬细胞活性的影响;Griess试剂检测K310对一氧化氮(NO)的影响;酶联免疫吸附法(ELISA)检测K310对IL-6炎性因子的影响;荧光定量PCR方法检测K310对诱导型一氧化氮(iNOS)和IL-6基因表达的影响;荧光免疫印迹法(Western bolt)检测K310对磷酸化的NF-kB和ERK1/2蛋白的影响。结果 K310(5、10和20μM)不影响LPS刺激RAW264.7巨噬细胞的活性;与LPS刺激组比较,K310不仅能显著抑制NO和IL-6的炎性因子及炎性基因;还能抑制磷酸化的NF-kB和ERK1/2蛋白的表达。结论 K310对LPS诱导RAW264.7巨噬细胞的炎症具有抗炎作用,其作用机制可能是K310通过抑制NF-kB和MAPK信号通路,从而抑制NO和IL-6炎性因子的产生。Objective To investigate the anti-inflammatory effect and mechanism of K310 on lipopolysaccharide(LPS)-induced RAW264.7 macrophages.Methods Cell Counting Kit-8(CCK-8)was used to detect determine the effect of different concentration of K310 on the activity of RAW264.7 macrophages induced by LPS.The Griess reagent was used to detect the effect of K310 on nitric oxide(NO).The ELISA method was used to the effect of K310 on the inflammatory factors of IL-6.Fluorescence quantitative PCR was used to detect the effect of K310 on the expression of inducible nitric oxide synthase(iNOS)and IL-6.Western blot was used to detect the effect of K310 on phosphorylated NF-kB and phosphorylated ERK1/2 protein.Results K310 of 5μM,10μM and 20μM showed no effect on the activity of LPS-induced RAW264.7 macrophages.Compared with LPS-stimulated group,K310 significantly inhibited the inflammatory factors and mRNA levels of NO and IL-6,and it also inhibited the expression of phosphorylated NF-kB and ERK1/2 protein.Conclusion K310 has the anti-inflammatory effect on LPS-induced RAW264.7 macrophages and its mechanism may be inhibiting the signaling pathway of NF-kB and MAPK to inhibit the production of the inflammatory factors of NO and IL-6.

关 键 词：K310 RAW264.7巨噬细胞 脂多糖 一氧化氮 IL-6 

分 类 号：R965[医药卫生—药理学]