黄芩素抑制胃癌MGC-803细胞增殖和迁移  

Baicalein inhibits proliferation and migration of gastric cancer MGC-803 cells

作　　者：孙沛林 朴日龙[3] 王莹 任香善 陈丽艳 林贞花 朴英实 SUN Pei-lin;PIAO Ri-long;WANG Ying;REN Xiang-shan;CHEN Li-yan;LIN Zhen-hua;PIAO Ying-shi(Cancer Research Center, Yanbian University,Yanbian 133000, China;Jilin Provincial Key Laboratory of Gynecological Tumor Bioinformatics, Yanbian 133000, China;Department of Functional Experimental Science, Yanbian University Medical College, Yanbian 133000, China)

机构地区：[1]延边大学肿瘤研究中心 [2]吉林省妇科肿瘤生物信息学重点实验室 [3]延边大学医学院机能实验中心,吉林延边133000

出　　处：《中国病理生理杂志》2018年第3期417-422,共6页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.31460294);吉林省教育厅"十三五"科学技术研究项目(吉教科合字[2016]第263号)

摘　　要：目的:探讨黄芩素(BAI)对胃癌MGC-803细胞增殖和迁移的作用及机制。方法:MGC-803细胞用不同浓度BAI处理后,采用MTT法检测细胞的存活率;平板集落形成实验检测细胞的集落形成能力;划痕愈合实验和Transwell小室迁移实验检测细胞的迁移能力;ELISA检测12-羟基二十碳四烯酸(12-HETE)的浓度;Western blot实验检测血小板型12-脂氧合酶(p12-LOX)、血管内皮生长因子(VEGF)、p-ezrin和上皮-间充质转化(EMT)标志物蛋白的表达。结果:BAI可显著抑制MGC-803细胞的增殖、平板集落形成及迁移(P<0.05或P<0.01),显著下调p12-LOX代谢产物12-HETE的浓度(P<0.05或P<0.01),并显著下调p12-LOX、VEGF、p-ezrin、vimentin和Snail蛋白的表达水平(P<0.05或P<0.01),上调E-cadherin蛋白的表达水平(P<0.01)。结论:BAI可有效抑制胃癌MGC-803细胞的增殖和迁移,其机制与BAI调控p12-LOX、VEGF、p-ezrin及EMT相关蛋白的表达变化有关。AIM:To investigate the effects of baicalein(BAI)on the proliferation and migration of gastric cancer MGC-803cells and the mechanisms.METHODS:After MGC-803cells were treated with BAI at different concentrations,the viability of the MGC-803cells was tested by MTT assay.The cell colony formation ability were detected by plate colony formation assay.Wound-healing and Transwell cell migration assays were used to test the migration ability of the MGC-803cells.The concentration of12-hydroxyeicosatetraenoic acid(12-HETE)was measured by ELISA.The protein levels of platelet type12-lipoxygenase(p12-LOX),vascular endothelial growth factor(VEGF),p-ezrin and epithelial-mesenchymal transition(EMT)markers in MGC-803cells were determined by Western blot.RESULTS:BAI significantly inhibited the proliferation,plate colony formation and migration abilities of the MGC-803cells(P<0.05or P<0.01),down-regulated the concentration of p12-LOX metabolite12-HETE significantly(P<0.05or P<0.01),decreased the protein levels of p12-LOX,VEGF,p-ezrin,vimentin and Snail(P<0.05or P<0.01),and increased the protein expression of E-cadherin(P<0.01).CONCLUSION:BAI suppresses the proliferation and migration abilities of gastric cancer MGC-803cells effectively.These effects of BAI may be related to regulating the protein levels of p12-LOX,VEGF,p-ezrin and EMT-related proteins.

关 键 词：黄芩素 胃癌 细胞增殖 细胞迁移 

分 类 号：R285.5[医药卫生—中药学] R730.23[医药卫生—中医学]