吴茱萸和臭辣吴萸果实化学成分及不同采收时间主要成分含量的变化  

Chemical constituents in fruits of Evodia rutaecarpa and E.fargesii,and changes in contents of main constituents at different harvesting times

作　　者：李懿恒 黄佳楠 刘潇 印敏[1] 刘飞[1] 冯煦[1] 王奇志[1] LI Yiheng;HUANG Jianan;LIU Xiao;YIN Min;LIU Fei;FENG Xu;WANG Qizhi(Jiangsu Provincial Key Laboratory for the Research and Utilization of Plant Resources, Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China)

机构地区：[1]江苏省中国科学院植物研究所(南京中山植物园)江苏省植物资源研究与利用重点实验室江苏省农业种质资源保护与利用平台,江苏南京210014

出　　处：《植物资源与环境学报》2018年第1期112-114,共3页Journal of Plant Resources and Environment

基　　金：国家自然科学基金资助项目(31470425;31570359);江苏省科技计划项目(BM2015019)

摘　　要：吴茱萸〔Evodia rutaecarpa(Juss.) Benth.〕及其变种石虎〔E.rutaecarpa var.officinalis(Dode) Huang〕和疏毛吴茱萸〔E.rutaecarpa var.bodinieri(Dode) Huang〕的干燥近成熟果实均为中药材吴茱萸的原植物,可散寒止痛、降逆止呕、助阳止泻,其天然成分对心血管系统、胃肠道系统和免疫系统也有一定功效[1]171,[2-3]。吴茱萸果实主要含生物碱、苦味素和挥发油等[4],吴茱萸碱、吴茱萸次碱和柠檬苦素含量是吴茱萸药材质量的评价标准[1]172。近年来,对吴茱萸的研究多集中于其不同产地和品种的药用成分研究[5-6]。同属的臭辣吴萸(E.fargesii Dode)果实在湖北民间用作吴茱萸代品,具相同药效[7]。为进一步明确吴茱萸和臭辣吴萸果实的化学成分,弄清药用有效成分随采收时间的变化规律,作者对吴茱萸和臭辣吴萸果实的化学成分进行了分离和鉴定,并分析了不同采收时间二者果实中主要成分含量的变化。Chemical constituents in fruits of Evodia rutaecarpa(Juss.)Benth.and E.fargesii Dode were identified by UPLC-Q-TOF-MS technology,and changes in contents of main constituents at different harvesting times were analyzed.The results show that common constituents in fruits of E.rutaecarpa and E.fargesii are thirteen,including indoloquinazolines,limonin and quinolones.Contents of main constituents in their fruits firstly increase and then decrease with the fruit maturing process.In fruits of E.rutaecarpa,contents of indoloquinazolines(including evodiamine,rutaecarpine,and dehydroevodiamine),limonin,and quinolones peak on September5th,August24th,and August14th,with1.5097%,0.4292%,and0.1896%,respectively;in fruits of E.fargesii,contents of three kinds of constituents peak on August14th,August4th,and July17th,with0.2152%,0.5211%,and0.2159%,respectively.The comprehensive analysis shows that the optimum harvesting time of E.rutaecarpa and E.fargesii is the end of August to early September and the end of July to the middle of August,respectively;total content of three kinds of constituents in fruits of E.rutaecarpa is higher than that of E.fargesii,and its medicinal quality is slightly better than the latter.

关 键 词：吴茱萸碱 成熟果实 化学成分 采收时间 心血管系统 ARPA 药用有效成分 吴茱萸次碱 

分 类 号：Q946.8[生物学—植物学] R284[医药卫生—中药学]