东湖表层沉积物中氨氧化古菌和氨氧化细菌丰度及多样性研究  

ABUNDANCE AND DIVERSITY OF AMMONIA-OXIDIZING ARCHAEA AND AMMONIA-OXIDIZING BACTERIA IN THE SURFACE SEDIMENTS OF DONGHU LAKE

作　　者：潘彦羽 代嫣然[1] 王飞华[1] 梁威[1] PAN Yan-Yu;DAI Yan-Ran;WANG Fei-Hua;LIANG Wei(State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Science, Wuhan 430072, China;University of Chinese Academy of Sciences, Beijing 100049, China)

机构地区：[1]中国科学院水生生物研究所淡水生态与生物技术国家重点实验室,武汉430072 [2]中国科学院大学,北京100049

出　　处：《水生生物学报》2018年第2期406-415,共10页Acta Hydrobiologica Sinica

基　　金：国家科技支撑计划课题(2015BAD13B02);湖北省科技厅面上基金项目(2015CFB275);浙江省科技厅项目(2015C32011)资助~~

摘　　要：研究通过高通量测序和荧光定量PCR等分子生物学分析方法,以氨单加氧酶基因(amo A)为分子标记,研究了东湖表层沉积物中AOA和AOB的群落多样性、丰度及其与环境因子的关系。结果表明,东湖沉积物AOA主要为Nitrosopumilus,其群落结构与沉积物中总氮含量显著相关,而AOB主要为Nitrosomonas,群落结构与沉积物中总有机碳和总磷显著相关。此外,不同季节AOA丰度均高于AOB,且沉积物AOA数量与温度呈显著负相关,但AOB丰度变化不明显。东湖沉积物中AOA可能主导了氨氧化过程。Ammonia oxidation is the rate-limiting step in nitrification.Ammonia-oxidizing archaea(AOA)and ammonia-oxidizing bacteria(AOB)play important roles in ammonia oxidation.Sediments are important place for nitrogencirculation in lake.This study investigated the community diversity and abundance of AOA and AOB in the surfacesediments of Donghu Lake and their relationships with environmental factors utilizing high-throughput sequencing andreal-time quantitative PCR of ammonia monooxygenase(amoA)gene.The results showed that AOA and AOB weredominated by Nitrosopumilus and Nitrosomonas,respectively.There were significant correlations between the communitystructure of AOA and total nitrogen(TN)in sediments.AOB were significantly related to the contents of totalorganic carbon(TOC)and total phosphorus(TP).Additionally,AOA abundance was higher than that of AOB in thesediments.A significantly negative correlation between AOA abundance and temperature was detected,but there wasno obvious change of AOB among the different seasons.These results implied that AOA could be the dominant driver in the ammonia oxidation in Donghu Lake.

关 键 词：氨氧化古菌 氨氧化细菌 东湖 群落结构 丰度 

分 类 号：Q938.15[生物学—微生物学]