丹参川芎嗪对兔耳增生性瘢痕的抑制作用研究  

Inhibitory Effect of Salviae Miltiorrhizae and Ligustrazine Hydrochloride Injection on Hypertrophic Scars of Rabbit Ears

作　　者：冯登超[1] 高栋梁[1] 白翠翠[1] 高东东[1] 武斌[1] 薛宏斌[1] 杨喜明[1] Feng Dengchao;Gao Dongliang;Bai Cuicui;Gao Dongdong;Wu Bin;Xue Hongbin;Yang Ximing(Department of Bums,Plastic and Hand Surgery,Yan'an University Affiliated Hospital,Yan'an,Shaanxi,China 716000)

机构地区：[1]延安大学附属医院烧伤整形手外科,陕西延安716000

出　　处：《中国药业》2018年第5期1-3,共3页China Pharmaceuticals

基　　金：陕西省自然科学基础研究计划项目[2014JM4199]

摘　　要：目的观察丹参川芎嗪注射液对兔耳增生性瘢痕的抑制作用。方法取日本大耳白兔16只,建立兔耳增生性瘢痕动物模型。待创面上皮化(伤后21 d)后将其随机分为4组,每组4只,对照组(A组)局部注射生理盐水,实验组(B,C,D组)分别局部注射低、中、高质量浓度的丹参川芎嗪注射液。于伤后32 d和56 d测量瘢痕厚度,并常规做HE染色,氯胺-T法检测瘢痕组织中羟脯氨酸(HPr)的含量变化;免疫组化观察瘢痕组织中增殖细胞核抗原蛋白(PCNA)的表达及变化。结果实验组中瘢痕厚度、HPr的含量及PCNA的阳性表达率与药物质量浓度呈负相关(P<0.05),瘢痕组织中胶原含量和PCNA蛋白水平均明显减少,呈剂量效应关系,与对照组比较,差异有统计学意义(P<0.01),伤后32 d和56 d实验组各亚组间差异有统计学意义(P<0.01)。结论丹参川芎嗪注射液通过减少胶原的合成水平,减少PCNA蛋白的表达,从而抑制兔耳增生性瘢痕组织增生,可为治疗增生性瘢痕提供新选择。Objective To observe the inhibitory efect of Salviae Miltiorrhizae and Ligustrazine Hydrochloride Injection on hypertrophicscars of rabbit ears.Methods Totally16healthy Japanese white big-ear rabbits were selected to establish animal model of hypertrophicscars of rabbit ears.After the wound epithelialization(21d after injury),the rabbits were randomly divided into4groups,4rabbitsin each group.The control group(group A)was partially injected with normal saline,and the experimental groups(group B,C,D)were partially injected with low,medium and high concentration of Salviae Miltiorrhizae and Ligustrazine Hydrochloride Injection.Scarthickness was measured on the32nd and56th day after injury,and HE staining was routinely performed,the content of HPr in scartissue was determined by chloramine-T method.The expression and changes of proliferating cell nuclear antigen(PCNA)in scar tissuewere observed by immunohistochemistry.Results In the experimental group,the thickness of scar,the content of HPr and the positiveexpression rate of PCNA were negatively correlated with the drug concentration(P<0.05).The collagen content and PCNA proteinlevel in the scar tissue of the experimental group were significantly decreased,showing a dose-response relationship,and the differencewas statistically significant compared with the control group(P<0.01).In the experimental group,there was significantdiffe re nee amongthe subgroups on the32nd and56th days after injuryP<0.01)Conclusion Salviae Miltiorrhizae and Ligustrazine HydrochlorideInjection can inhibit the proliferation of hypertrophic scar tissue in rabbit ears by reducing the level of collagen synthesis and the expressionof PCNA protein,which can provide new options for the treatment of hypertrophic scars.

关 键 词：丹参川芎嗪注射液 增生性瘢痕 增殖细胞核抗原 兔耳 

分 类 号：R285.5[医药卫生—中药学] R269[医药卫生—中医学]