针刺对失眠大鼠血清去甲肾上腺素、多巴胺及5-羟色胺含量的影响  

Effects of Acupuncture on 5-HT, NE and DA in Serum of Insomnia Rats

作　　者：郭鑫[1] 岳增辉[1] 谢菊英 吴雪芬[1] 郑雪娜[1] GUO Xin;YUE Zeng-hui;XIE Ju-ying;WU Xue-fen;ZHENG Xue-na(Hunan University of Chinese Medicine, Changsha 410208, China)

机构地区：[1]湖南中医药大学,湖南长沙410208

出　　处：《中国中医药信息杂志》2018年第4期46-50,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(2014CB543102);国家自然科学基金(81673886)

摘　　要：目的观察循经选穴针刺对失眠大鼠外周血清单胺类神经递质5-羟色胺(5-HT)、多巴胺(DA)、去甲肾上腺素(NE)含量及腧穴配伍效应的影响,探讨针刺治疗失眠症的作用机制。方法将60只SD大鼠随机分为空白组、模型组、百会+神门组、百会+三阴交组和百会+非经非穴组,每组12只,腹腔注射对氯苯丙氨酸混悬液制作大鼠失眠模型,各针刺组针刺相应腧穴,每次30 min,连续7 d。ELISA检测大鼠血清NE、DA、5-HT的含量。结果与空白组比较,模型组大鼠血清5-HT、DA、NE含量明显升高(P<0.01);与模型组比较,各针刺组大鼠血清DA、NE含量明显降低(P<0.05,P<0.01),百会+三阴交组大鼠血清5-HT含量明显降低(P<0.05);各针刺组间比较,百会+神门组总体疗效优于百会+三阴交组及百会+非经非穴组。结论针刺改善大鼠睡眠结构可能与血清单胺类神经递质含量有关,循经选穴针刺可能是影响腧穴配伍效应的因素之一。Objective To observe the effects of acupuncture in acupoints at different meridians on5-HT,DA,NE in the peripheral serum of insomnia rats;To explore the mechanism of action of acupuncture for the treatment of insomnia.Methods Sixty SD rats were randomly divided into blank group,model group,Baihui+Shenmen group,Baihui+Sanyinjiao group,and Baihui+non-acupoint group,with12rats in each group.Injection of PCPA suspension of rats was used to establish insomnia model.Each acupuncture group received corresponding acupoints,30min each time,for7days.The levels of5-HT,DA and NE in serum were measured by ELISA.Results Compared with the blank group,the levels of5-HT,DA and NE in the model group were significantly higher than those in the blank group(P<0.01).Compared with the model group,the contents of DA and NE in the acupuncture groups were significantly lower than those in the model group(P<0.05,P<0.01),and the5-HT in Baihui+Sanyinjiao group was significantly lower than those in the model group(P<0.05).Among all acupuncture groups,overall efficacy of Baihui+Shenmen group was better than Baihui+Sanyinjiao group and Baihui+non-acupoint group.Conclusion Acupuncture can improve sleep structure in rats may be related to serum monoamine neurotransmitters.Acupuncture in acupoints at different meridians may be one of the influencing factors of acupoint compatibility effects.

关 键 词：针刺 失眠 循经选穴 去甲肾上腺素 多巴胺 5-羟色胺 大鼠 

分 类 号：R245[医药卫生—针灸推拿学]