当归多糖对阿尔茨海默病模型大鼠学习记忆及β-淀粉样蛋白代谢的影响  

Effects of Angelica Polysaccharide on Learning and Memory Abilities and Aβ Metabolism in Model Rats with Alzheimer Disease

作　　者：王虎平[1] 吴红彦[1] 李海龙[1] 马春林[1] 曾庆涛[1] 崔淑梅 朱凯敏 WANG Hu-ping;WU Hong-yan;LI Hai-long;MA Chun-lin;ZENG Qing-tao;CUI Shu-mei;ZHU Kai-min(Gansu University of Chinese Medicine, Key Laboratory of Traditional Chinese Herbs Discovery and Innovation and Transformation in Gansu Province, Lanzhou 730000, China)

机构地区：[1]甘肃中医药大学,甘肃省方药挖掘与创新转化重点实验室,甘肃兰州730000

出　　处：《中国中医药信息杂志》2018年第4期51-55,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：甘肃省自然科学基金(1212RJZA075);甘肃省创新研究群体计划(1308RJIA001);甘肃省重点产业创新能力建设专项资金(2013年)

摘　　要：目的观察当归多糖对阿尔茨海默病(AD)模型大鼠学习记忆、海马β-淀粉样蛋白前体(APP)、β-淀粉样蛋白(Aβ)1-42及血清乙酰胆碱(Ach)、胆碱乙酰转移酶(ChAT)、乙酰胆碱酯酶(AChE)、超氧化物歧化酶(SOD)、丙二醛(MDA)的影响,探讨其防治AD的作用机制。方法 70只SPF级Wistar大鼠经水迷宫学习记忆能力筛选合格后,随机选取10只大鼠(雌雄各半)为假手术组,其余大鼠以脑立体定位注射Aβ25-35复制AD大鼠模型,以水迷宫学习记忆能力筛选造模成功的50只大鼠随机分为模型组、阳性药组和当归多糖低、中、高剂量组,每组10只。模型组和假手术组大鼠给予生理盐水灌胃,各给药组大鼠给予相应药液灌胃,每日给药体积均为2 m L/100 g,连续28 d。给药25~28 d Morris水迷宫测试大鼠学习记忆能力,然后取材检测血清Ach、ChAT、AChE、SOD、MDA及海马APP、Aβ1-42。结果与假手术组比较,模型组大鼠定位航行实验逃避潜伏期明显延长,目标象限滞留时间缩短,空间探索实验首次到达原逃生平台位置潜伏时间延长,穿越原平台位置及目标象限滞留的时间缩短,血清Ach含量与ChAT、SOD活性明显降低,AChE活性及MDA水平明显升高,海马APP、Aβ1-42含量升高,差异均有统计学意义(P<0.05,P<0.01);与模型组比较,各给药组大鼠逃避潜伏期均不同程度缩短,目标象限滞留时间延长,首次到达原逃生平台位置潜伏时间缩短,跨原平台次数增加,血清Ach含量和ChAT、SOD活性升高,AChE活性及MDA水平明降低,海马APP、Aβ1-42含量降低,差异均有统计学意义(P<0.05,P<0.01)。结论当归多糖可能通过改善胆碱能神经递质、提高抗自由基氧化能力及促进Aβ的代谢,改善AD模型大鼠学习记忆能力,对AD有一定的防治作用。Objective To investigate effects of angelica polysaccharide on learning and memory abilities,Ach,ChAT,AChE,SOD,MDA in serum,APP and Aβ1-42in hippocampus in model rats with Alzheimer disease(AD);To explore the mechanism of angelica polysaccharide for the treatment of AD.Methods Seventy SPF Wistar rats were selected for learning and memory ability by water maze.10rats were randomly selected(half female and half male)assham-operation group,and the others were injected with Aβ25-35by stereotatic techniques,copying AD model rats.50rats for learning and memory ability by water maze were successfully divided into model group,positive group,angelica polysaccharide low-,medium-,and high-dose groups,with10rats in each group.Rats in model group and sham-operation group were given normal saline for gavage,while rats in medication groups were given relevant medicine for gavage,2mL/(100g?d),for28d.The learning and memory ability of rats in each group was tested by Morris water maze during25-28days,and the contents of Ach,ChAT,AChE,SOD,MDA in serum and APP and Aβ1-42in hippocampus were determined.Results Compared with the sham-operation group,the escape latent period of model group was significantly prolonged in place navigation experiment;the target quadrant time was shortened;the latent time for the first time to reach the original escape platform was longer in spatial probe test;the residence time of crossing the original platform position and the target quadrant was shorter;the levels of Ach,the activity of ChAT and SOD in serum decreased;the levels of MDA,the activity of AChE in serum increased;the levels of APP and Aβ1-42in hippocampus increased,with statistical significance(P<0.05,P<0.01).Compared with model group,the escape latent period of each medication group was shortened in different degrees after the intervention treatment;the residence time of target quadrant was prolonged;the latent time for the first time to reach the original escape platform was shortened;the number of cross platform increased;the levels o

关 键 词：当归多糖 MORRIS水迷宫 神经递质 抗氧化 -淀粉样蛋白前体 -淀粉样蛋白1-42 大鼠 

分 类 号：R285.5[医药卫生—中药学]