壳寡糖锌配合物的制备及抑菌活性  

Preparation and Antibacterial Activity of the Chitosan Oligosaccharide-Zn^(2+) Complexes

作　　者：卢琪洁 王丽 钱旭 朱雨晴 许钲 张宾 LU Qi-jie;WANG Li;QIAN Xu;ZHU Yu-qing;XU Zheng;ZHANG Bin(Key Laboratory of Health Risk Factors for Seafood of Zhejiang Province, College of Food and Medicine,Zhejiang Ocean University, Zhoushan 316022, Zhejiang, China)

机构地区：[1]浙江海洋大学食品与医药学院浙江省海产品健康危害因素关键技术研究重点实验室,浙江舟山316022

出　　处：《食品研究与开发》2018年第6期58-65,共8页Food Research and Development

基　　金：中国博士后基金项目(2016M590663;2017T100518);浙江省自然科学基金项目(LY18C200008);浙江海洋大学大学生生命科学竞赛项目

摘　　要：探索壳寡糖锌配合物的制备工艺及评价其抑菌活性。根据Box-Behnken试验原理,以壳寡糖(chitosan oligosaccharide,COS)与Zn^(2+)配比、体系pH值、反应温度及时间为影响因素,以对金黄色葡萄球菌和大肠杆菌的抑制活性作为响应值,进行壳寡糖锌(COS-Zn^(2+))制备工艺响应面优化分析。结果发现,COS-Zn^(2+)制备条件对其抑菌活性影响从大到小依次为:COS与Zn^(2+)配比>反应温度>反应时间>体系pH值,进而获得最佳制备工艺参数:pH5,反应时间2 h,糖盐比例1:1(质量比),反应温度45℃。在此参数条件下,制备COS-Zn^(2+)对金黄色葡萄球菌和大肠杆菌的抑菌圈直径为33.08 mm和32.23 mm,与建立模型的预测值基本相符。研究结果可为海洋壳寡糖资源生物活性开发及应用,提供一定的参考与理论基础。The present experiment was conducted to investigate the preparation of chitosan oligosaccharide-zinc(COS-Zn2+)complex and focused primarily on its antibacterial activity.According to Box-Behnken experimental principle,the response surface methodology was performed,with the ratio of COS to Zn2+,pH,reaction temperature,and time as the experimental factors,versus the inhibitory activities against S.aureus and E.coli as response value.The results indicated the influence of preparation factors on antibacterial activity was as follows:the ratio of COS to Zn2+,temperature,time,and pH,respectively.Then,the optimum preparation conditions were obtained,which the value of pH was5,the reaction time was2h,the ratio of COS to Zn2+was1∶1(mass ratio),and the temperature was45℃,respectively.Under these conditions,the inhibition diameters of prepared COS-Zn2+on S.aureus and E.coli were33.08mm and32.23mm,respectively,which were almost accorded with the predicted data.These results could provide a reference and theoretical basis for the development and application of marine chitosan oligosaccharide resources.

关 键 词：壳寡糖 锌 响应面分析 大肠杆菌 金黄色葡萄球菌 

分 类 号：O641.4[理学—物理化学] TS201.2[理学—化学]