基于镉特异性功能化核酸适配体测定痕量镉的共振光散射新方法  

A new resonance light scattering method based on cadmium specific functional aptamer for detection of trace cadmium

作　　者：唐律[1] 李贵荣[1] 徐梦媛[1] 杨洋 TANG Lü;LI Gui-rong;XU Meng-yuan;YANG Yang(College of Public Health,University of South China,Hengyang 421001,China)

机构地区：[1]南华大学公共卫生学院,湖南衡阳421001

出　　处：《应用化工》2018年第3期627-630,共4页Applied Chemical Industry

基　　金：湖南省教育厅重点课题(14A123);湖南省科技厅科技计划重点课题(2015SK2001)

摘　　要：镉特异性功能化核酸适配体(FA_(Cd))在无镉体系中能阻碍金纳米(GNPs)与罗丹明6G(Rh6G)聚集,体系共振光散射强度较低。当体系中存在镉离子时,FACd与之特异性结合,从RNA位点处断裂产生适配体片段(FAF),FAF与GNPs和Rh6G形成GNPs-FAF-Rh6G聚合物,共振光散射强度大幅增加,共振光散射强度值(I_(RLS))与镉的浓度(cCd^(2+))在0.28~11.2 ng/mL范围内呈现良好线性关系,检出限为0.04 ng/mL,相对标准偏差≤5%,加标回收率为96.4%~101.4%。Cadmium specific functional aptamer(FACd)can hinder gold nanoparticles and Rhodamine 6G from gathering in a system without cadmium,keep system’s resonant light scattering at a low level.When cadmium exist in system,it can specific binding with FACd and activating enzyme activity of FACd,FACd break from the RNA site,fragments(FAF)form large size Rh6G-FAF-GNPs polymer with Rh6G and GNPs,resulting a greatly increase of system’s resonant light scattering.RLS has a good linear relationship with cadmium ion concentration in range of 0.28~11.2 ng/mL,limit of detection is 0.04 ng/mL,relative standard deviation is less than 5%,adding standard recovery is 96.4%~101.4%.

关 键 词：镉特异性功能化核酸适配体 金纳米 罗丹明 共振光散射法 镉 

分 类 号：TQ581[化学工程—精细化工] R115[医药卫生—公共卫生与预防医学]