示指与接触物菌群ERIC-PCR指纹图谱比对  

Comparison of Bacteria ERIC-PCR Fingerprints of Index Fingers and Contactants

作　　者：刘耘汀 孙大明[1] 施少培 杨旭 LIU Yun-ting;SUN Da-ming;SHI Shao-pei;YANG Xu(East China University of Political Science and Law, Shanghai 200043, China;Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China)

机构地区：[1]华东政法大学,上海200043 [2]司法鉴定科学研究院,上海市法医学重点实验室,上海市司法鉴定专业技术服务平台,上海200063

出　　处：《法医学杂志》2018年第1期33-36,共4页Journal of Forensic Medicine

基　　金：国家社会科学基金资助项目(17BFX063);上海市法医学重点实验室资助项目(17DZ2273200);上海市司法鉴定专业技术服务平台资助项目(16DZ2290900)

摘　　要：目的探讨示指菌群和接触物(手机触摸屏、个人办公桌桌面)表面菌群之间的关联性。方法采集10名志愿者的示指、手机触摸屏、个人办公桌桌面的菌群,通过宏基因组PCR扩增技术建立肠道细菌基因间重复序列(enterobacterial repetitive intergenic consensus,ERIC)-PCR指纹图谱。结果 7名志愿者的示指与手机触摸屏菌群ERIC-PCR指纹图谱对应吻合,且各志愿者个体之间ERIC-PCR指纹图谱互不相同;3名志愿者的示指菌群与个人办公桌桌面菌群ERIC-PCR指纹图谱对应吻合,其余7名志愿者的示指菌群与个人办公桌桌面菌群ERIC-PCR指纹图谱不完全吻合,但分别存在大小一致的条带。结论示指所携带的细菌菌群具有一定个体特异性,示指接触手机触摸屏、办公桌桌面后残留下来的菌群,可作为潜在的法医学鉴定的新型生物样本。Objective To explore the bacteria relevance between index fingers and contactant’surfaces(mobile phone touch screen and desktop of personal office table).Methods Bacteria were collected from the index fingers,mobile phone touch screen and desktop of personal office table of10volunteers.Enterobacterial repetitive intergenic consensus(ERIC)-PCR fingerprint was established by PCR amplification technique of metagenome.Results There were7volunteers’ERIC-PCR fingerprints of index fingers matched that took from the mobile phone touch screens,and different from each other.There were3volunteers’ERIC-PCR fingerprints of index fingers matched that took from desk top of personal office table,and other7volunteers’ERIC-PCR fingerprints did not match perfectly with that took from desk top of personal office table,but had at least one similar band for both.Conclusion The bacteria on index finger shows individual specificity,which on mobile phone touching screen and personal desktop may be a new biological sample of forensic identification.

关 键 词：法医遗传学 聚合酶链反应 DNA指纹法 肠道细菌基因间重复序列 指纹图谱 

分 类 号：DF795.2[医药卫生—法医学]