西部煤炭资源地下开采对不同坡位柠条光合系统损伤特征分析  

Damage Characteristics of Caragana korshinskii Microphylla Photosynthetic System at Different Slope Positions in the Underground Coal Mining of Western China

作　　者：王丽[1,2] 雷少刚[1] 卞正富[1] WANG Li;LEI Shaogang;BIAN Zhengfu(School of Environment Science and Spatial Informatics,China University of Mining and Technology,Xuzhou, Jiangsu221116,China;School of Geography Information and Tourism,Chuzhou University,Chuzhou, Anhui 239000,China)

机构地区：[1]中国矿业大学环境与测绘学院,江苏徐州221116 [2]滁州学院地理信息与旅游学院,安徽滁州239000

出　　处：《西北植物学报》2018年第2期332-342,共11页Acta Botanica Boreali-Occidentalia Sinica

基　　金："十三五"国家重点研发计划(2016YFC0501107);国家自然科学基金重点项目(U1361214)

摘　　要：以神东采煤沉陷区不同坡位(坡上、坡中、坡下)的柠条植株为目标植物,应用叶绿素荧光诱导动力学及分析技术(JIP-test),比较分析沉陷后1~2年不同坡位柠条叶绿素荧光参数的变化特征,探索不同坡位柠条PSⅡ(光系统Ⅱ)损伤及适应机制的空间差异性,为矿区植被恢复与重建提供理论指导。结果显示:(1)坡上和坡下柠条叶片J点相对可变荧光(V_j)和Q_A的初始还原速度(M_o)明显增加,同时单位反应中心吸收的光能(ABS/RC)、捕获的光能(TR_o/RC)和单位面积热耗散的能量(DI_o/CS_o)增大,最大光化学效率(F_v/F_m)和以吸收光能为基础的性能指数(PI_(ABS))大幅减少,K相可变荧光占J的比例(W_k)在沉陷1年最大,增幅达74.54%,沉陷2年后逐渐接近对照值。(2)坡中柠条叶片V_j和M_o先增加后减少,ABS/RC、TR_o/RC明显减小,F_v/F_m、PIAB S和W_k变化不大。(3)冗余分析(redundancy analysis,RDA)前2个排序轴共同解释了荧光参数-环境因子间关系的82.98%,其中土壤水和速效磷与第一轴的相关系数分别为0.57和0.50。研究表明:采煤塌陷后坡上和坡下柠条叶片PSⅡ受损严重,反应中心通过耗散过剩的激发能减轻伤害;坡中柠条受PSⅡ采煤塌陷影响较小,反应中心通过可逆性失活进行自我保护;土壤水和速效磷含量是采煤沉陷区制约柠条PSⅡ光合生理的关键环境因子。Using chlorophyll fluorescence induction kinetics and analysis technique(JIP-test),the study analyzed the damage status of plants and the photosynthetic response of plants to stress.The result is very important to vegetation restoration and reconstruction in mining area.Taken Caragana korshinskii as the target plant,which live in Shendong Mining subsidence area of different slope positions(upslope,mesoslope and downslope).The study compared the differences in chlorophyll fluorescence parameters from1to2years after coal ming subsidence.In this paper,it has explored that the degree of damage and adaptability mechanism of different slope positions in coal mining subsidence.The results show that:(1)on upslope and downslope,V j and M o of C.korshinskii leaves increased significantly;at the same time,ABS/RC,TR o/RC and DI o/CS o increased,while F v/F m and PI ABS decreased greatly;after1years of subsidence,the maximum increase of W k value reached74.54%,and it was close to the control value after2years of subsidence.(2)On mesoslope,V j and M o of C.korshinskii increased first and then decreased,while ABS/RC and TR o/RC decreased significantly,F v/F m,PI AB S and W k changed little.(3)The first two axes of redundancy analysis effectively explained52%of Fluorescence-environmental variation.The correlation coefficients of soil water and available phosphorus with the first axis were0.57and0.5,respectively.It suggest that the photosynthetic systemⅡ(PSⅡ)of Caragana was severely damaged in the upper and lower slopes,and the reaction center reduced damage by dissipating excess excitation energy;the PSⅡof Caragana in middle slope was less affected by coal mining,and the reaction center protected itself by reversible inactivation;soil water,available phosphorus and available potassium are the key environmental factors that affecting the PSⅡof Caragana in the coal mining subsidence area.

关 键 词：半干旱区 地下开采 柠条 叶绿素荧光 光合系统损伤 

分 类 号：Q945.79[生物学—植物学]