南瓜未授粉子房培养形成再生植株的研究  

In Vitro Culture of Unfertilized Ovary of Pumpkin and to Form Regenerated Plantlets

作　　者：邓英[1,2] 唐兵 吴康云 卢松[1,2] 文林宏 张万萍[3] DENG Ying;TANG Bing;WU Kangyun;LU Song;WEN Linhong;ZHANG Wanping(Institute of Horticulture, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China;Guizhou Horticultural Engineering Technology Research Center, Guiyang 550006, China;Agricultural College of Guizhou University, Guiyang 550025, China)

机构地区：[1]贵州省农业科学院园艺研究所,贵阳550006 [2]贵州省园艺工程技术研究中心,贵阳550006 [3]贵州大学农学院,贵阳550025

出　　处：《西北植物学报》2018年第2期381-385,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：贵州省蔬菜产业技术体系建设子项(GZCYX2014-01);国家自然科学基金(31460515);黔农科院院专项([2015]19号);黔农科院(CR[2014]23号);黔科合平台人才([2016]5671);黔科合人才([2013]42号);贵州大学引进人才科研项目([2011]09号);黔科合条中补地[2015]4002

摘　　要：以‘贵阳小青瓜’(南瓜)未授粉子房为外植体,在离体条件下研究热激时间和TDZ浓度对胚状体诱导的影响。进一步优化试验步骤,探讨直接获得形成再生植株的方法,加速育种进程。结果表明:35℃高温黑暗培养5d后转入25℃光照培养90d能直接形成再生植株;培养基中附加TDZ浓度在0.04~0.06mg/L能诱导离体子房直接产生再生植株,并以0.06mg/L诱导效果最好,出胚率为22.57%,植株再生率为14.30%。We used unfertilized ovaries of‘Guiyang xiaoqinggua’(pumpkin)as the explants to study the heat shock time and TDZ concentration on embryo induction via in vitro culture.Furthermore,we optimized the test steps and researched the method of obtaining regenerated plants directly.The results showed that the best obtained pumpkin plants were inducted regenerated plants directly under dark at35℃for5d,and then moved to light condition at25℃for90d;The unfertilized ovaries can induct regenerated plants in the TDZ concentration at0.04-0.06mg/L,the best TDZ concentration was0.06mg/L,and the ratio of inducted embryo and regenerated plants were22.57%and14.30%,respectively.

关 键 词：南瓜 未受粉子房 胚状体 再生植株 

分 类 号：Q813.13[生物学—生物工程]