玉屏风散加味方中“涤痰”“逐瘀”作用药对对COPD大鼠肺组织TGF-β_1/Smad通路的影响  

The Influence of Couplet Medicines of ″Washing Away the Phlegm″ and ″Expelling the Stasis″ in Modified YuPingFeng Powder on TGF-β_1/ Smad Pathwayin Lung Tissue of COPD Rat Model

作　　者：席晓蓉[1] 程羽[1] 羊忠山 赵文娟[1] 潘晶晶[1] 袁嘉丽[1] XI Xiaorong;CHENG Yu;YANG Zhongshan;ZHAO Wenjuan;PAN Jingjing;YUAN Jiali(Yunnan University of TCM, Kunming 650500, China)

机构地区：[1]云南中医学院,云南昆明650500

出　　处：《西部中医药》2018年第3期9-11,共3页Western Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(编号81460684);云南省教育厅重大项目(编号2014Z121)

摘　　要：目的:探讨玉屏风散加味方中"涤痰"和"逐瘀"作用药对对慢性阻塞性肺疾病(COPD)大鼠TGF-β_1/Smad信号转导通路的影响。方法:将雄性SPF级Wistar大鼠90只随机分为正常组、模型组、罗红霉素组及"涤痰"作用药对高、中、低剂量组和"逐瘀"作用药对高、中、低剂量组。采用酶联免疫吸附试验(ELI SA)检测大鼠转化生长因子β_1(TGF-β_1),采用蛋白质印迹法(Western blot)检测大鼠肺组织Smad2/3、Smad7表达水平。结果:"涤痰"作用药对和"逐瘀"作用药对低、中剂量组及罗红霉素组TGF-β_1表达水平较模型组降低;"涤痰"作用药对低剂量组Smad2/3水平低于模型组;"涤痰"作用药对高剂量组和"逐瘀"作用药对各剂量组Smad7水平高于模型组。结论:玉屏风散加味方中"涤痰"和"逐瘀"作用药对对COPD大鼠TGF-β_1/Smad信号转导通路有调节作用。Objective:To discuss the influence of couplet medicines of"washing away the phlegm"and"expelling the stasis"in modified YuPingFeng powder on TGF-β1/Smad signal transduction pathway in lung tissue of COPD rat model.Methods:Ninety Wistar male SPF rats were randomized into the normal group,the model group,roxithromycin group,high,moderate and low dose groups of couplet medicines of"washing away the phlegm"as well as high,moderate and low dose groups of couplet medicines of"expelling the stasis".The expressions of Smad2/3and Smad7in lung tissue of the rats were detected by Western blot,and the expression of TGF-β1by ELISA method.Results:The expression of TGF-β1in moderate and low dose groups of couplet medicines of"washing away the phlegm"and"expelling the stasis"and roxithromycin group was lower compared with the model group;low dose group of couplet medicines of"washing away the phlegm"was lower than the model group in the expressions of Smad2/3,high dose group of couplet medicines of"washing away the phlegm"and different doses groups of couplet medicines of"expelling the stasis"were higher than the model group in the levels of Smad7.Conclusion:Couplet medicines of"washing away the phlegm"and"expelling the stasis"in modified YuPingFeng powder could regulate TGF-β1/Smad signal transduction pathway in lung tissue of COPD rat model.

关 键 词：慢性阻塞性肺疾病 TGF-Β1/SMAD 信号转导通路 玉屏风散加味方 “涤痰” “逐瘀” 药对 

分 类 号：R285[医药卫生—中药学]