黄芪总苷对阿霉素诱导损伤足细胞的保护作用  

Protecting Effects of Total Astragalus Glycosides on Adriamycin-induced Podocytes Injury

作　　者：宋元春[1] 赛依帕[1] 高霞[1] 罗璇[1] SONG Yuanchun;SAI Yipa;GAO Xia;LUO Xuan(Pediatric Department, Gansu Provincial Hospital, Lanzhou 730000, China)

机构地区：[1]甘肃省人民医院儿科,甘肃兰州730000

出　　处：《西部中医药》2018年第3期19-22,共4页Western Journal of Traditional Chinese Medicine

基　　金：甘肃省中医药管理局项目(编号G2K-2014-59)

摘　　要：目的:探讨黄芪总苷保护阿霉素(ADR)诱导损伤足细胞的分子作用机制。方法:采用0.5μmol/L的ADR处理小鼠足细胞系(MPC5)条件永生型小鼠足细胞系24小时,建立足细胞损伤模型;以50、100、200μg/mL3个浓度的黄芪总苷分别处理ADR损伤足细胞24小时后,采用酶联免疫吸附法(ELISA)检测MMP2及MMP9表达水平;采用蛋白质印迹法(Western blot)检测各组凋亡相关蛋白表达水平及MT1、Nephrin蛋白质表达水平。结果:ELISA结果显示,基质金属蛋白酶(MMPs)表达水平受ADR抑制,各浓度黄芪总苷可改善该异常表达;蛋白质印迹实验结果显示,ADR可上调促凋亡相关蛋白表达水平,下调MT1、Nephrin及凋亡抑制相关蛋白的表达水平,各浓度黄芪总苷可显著改善各蛋白质的异常表达。结论:黄芪总苷对ADR损伤的MPC5足细胞具有保护作用,这可能与黄芪总苷可以下调ADR损伤足细胞Bax、P53蛋白表达水平并上调MMP-2、MMP-9、MT1及Nephr i n等蛋白表达水平有关。Objective:To discuss molecular mechanism of total astragalus glycosides(AST)protecting adriamycin(ADR)-induced podocytes injury.Methods:Podocyte injury model was established by dealing MPC5conditional immortality type podocyte series with ADR,0.5μmol/L,for24hours;the levels of MMP2and MMP9were detected by ELISA method after dealing ADR-induced podocyte with AST,50,100and200μg/mL for24hours respectively;the expressions of MT1,Nephrin protein and apoptosis related protein in different groups were measured by Western blot method.Results:ELISA results showed that the expressions of matrix metalloproteinase(MMPs)were inhibited by ADR,AST in different concentrations could improve the abnormal expressions;western blot methods indicated that ADR could up regulate the expressions of apoptosis-related protein,down regulate the expressions of MT1,Nephrin and apoptosis related protein,AST in different concentrations could improve the abnormal expressions of the protein.Conclusion:AST could protect ADR-induced MPC5podocyte,and it might be related to that AST could down regulate the expressions of Bax and P53protein,up regulate the expressions of MMP-2,MMP-9,MT1and Nephrin protein of ADR-induced podocyte injury.

关 键 词：足细胞 凋亡相关蛋白 基质金属蛋白酶 黄芪总苷 阿霉素 

分 类 号：R285.5[医药卫生—中药学]