黄花蒿叶水提物对三七根际尖孢镰刀菌生长的抑制作用  

Inhibitory Effects of Water Soluble Extract from Artemisia annua Leaves on The Growth of Rhizosphere Fusarium oxysporum Associated with Panax notoginseng

作　　者：赵雅萌[1] 马玉楠 陈传娇 程永现 董鲜[1] 徐福荣[1] ZHAO Ya-meng;MA Yu-nan;CHEN Chuan-jiao;CHENG Yong-xian;DONG Xian;XU Fu-rong(Yunnan University of Traditional Chinese Medicine,Kunming 650500,China;School of Pharmaceutical Sciences,Health Science Center,Shenzhen University,Shenzhen 518060,China)

机构地区：[1]云南中医学院,昆明650500 [2]深圳大学医学部药学院,深圳518060

出　　处：《天然产物研究与开发》2018年第3期373-378,353,共7页Natural Product Research and Development

基　　金：国家自然科学基金(81660626);国家自然科学基金(81460581);云南应用基础研究计划-青年项目(2015FD034);云南省应用基础研究-中医联合专项(2017FF116-014);国家重点研发计划(2017YFD0201402)

摘　　要：田间试验结果表明,在三七土壤表面覆盖黄花蒿可促进三七植株的生长,并且对三七"黄腐型"根腐病有一定的抑制作用,防控效果高达76.6%。通过以水为溶媒提取黄花蒿叶片,研究了其水提物对三七根腐病主要病原菌之一尖孢镰刀菌菌丝生长、孢子产量、菌丝干重、镰刀菌酸产生的作用。结果表明,随着水提物浓度的增大,黄花蒿水提物对尖孢镰刀菌菌丝的生长、孢子产量和镰刀菌酸产生的抑制作用逐渐增强;并且病原菌培养基的粗提物对植株体的致病作用与其镰刀菌酸含量成正相关关系。该研究将为三七根腐病的生态防控以及新农药的开发利用提供重要科学依据。The field tests showed that covering Artemisia annua L.on the Panax notoginseng’s soil surface can promote plant growth and meanwhile inhibit the occurrence of root-rot disease of P.notoginseng with preventive effect even up to 76.6%.To unveil the effect of water soluble extract of A.annua leaves on the growth of Fusarium oxysporum,a well-known pathogenic fungus of P.notoginseng,the colony diameter,spore yields,hypha dry weight and fusaric acid content were measured.It was found that the mycelium growth,spore yields,hypha dry weight and fusaric acid content significantly decreased in a dose dependent manner.In addition,the content of fusaric acid in medium was found to be positively correlated with the injury level of plant.The present study would lend supports for both ecological control and development of new pesticides of P.notoginseng.

关 键 词：黄花蒿 三七 尖孢镰刀菌 水提物 根腐病 

分 类 号：R282.2[医药卫生—中药学]