木麻黄凋落物化学成分及其生物活性的研究  

Chemical Constituents from Litters of Casuarina equisetifolia and Their Biological Activity

作　　者：王海生 戴好富[2] 王佩[2] 蔡彩虹[2] 周丽曼[2] 李蕾[1] 梅文莉[2] WANG Hai-sheng;DAI Hao-fu;WANG Pei;CAI Cai-hong;ZHOU Li-man;LI Lei;MEI Wen-li(Hainan Normal University,Haikou 571158,China;Key Laboratory of Biology and Genetic Resources of Tropical Crops,Ministry of Agriculture,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China)

机构地区：[1]海南师范大学,海口571158 [2]中国热带农业科学院热带生物技术研究所,农业部热带作物生物学与遗传资源利用重点实验室,海口571101

出　　处：《天然产物研究与开发》2018年第3期390-395,533,共7页Natural Product Research and Development

基　　金：国家自然科学基金(31260068);海南师范大学2016年研究生校级创新科研项目(Hsyx2016-24);海南自然科学基金创新研究团队项目(2017CXTD020)

摘　　要：利用多种柱色谱和高效液相色谱相结合的方法从木麻黄(Casuarina equisetifolia)凋落物的乙酸乙酯提取物中分离得到7个单体化合物,通过波谱分析结合理化性质鉴定化合物结构分别为:3β-(p-hydroxy-trans-cinnamoyloxy)olean-12-en-28-oicacid(1),3-O-(E)-coumaroylerythrodiol(2),casuarmondtol(3),alnusdiol(4),山柰酚-3-O-α-L-鼠李糖苷(5),4″-反-香豆酰基-山柰酚-3-O-α-L-鼠李糖苷(6),山柰酚(7),其中化合物3、4、6和7为首次从该植物中分离得到。分别采用MTT法、Ellman法和PNPG法对单体化合物的体外细胞毒活性、乙酰胆碱酯酶抑制活性和α-糖苷酶抑制活性进行测试,测试结果表明,化合物1和6具有细胞毒活性,化合物1~4和6具有乙酰胆碱酯酶抑制活性,化合物1和3~5具有α-糖苷酶抑制活性。Seven compounds were isolated from the ethyl acetate(EtOAc)extract of litters of Casuarina equisetifolia by the method of column chromatography and high performance liquid chromatography.Their structures were identified as 3β-(p-Hydroxy-trans-cinnamoyloxy)olean-12-en-28-oicacid(1),3-O-(E)-coumaroylerythrodiol(2),casuarmondtol(3),alnusdiol(4),kaempferol3-O-α-L-rhamnopyranoside(5),4′′-trans-p-coumaroyl-kaempferol-3-O-α-rhamnopyranoside(6)and kaempferol(7)on the basis of physicochemical properties and spectroscopic evidence.Compounds 3,4,6 and 7 were obtained from Casuarina equisetifolia for the first time.The compounds were tested for cytotoxicity,acetylcholinesterase andα-glucosidase inhibitory activities by MTT,Elman and PNPG methods,respectively.Compounds 1 and 6 showed cytotoxic activity.Compounds 1-4 and 6 showed acetylcholinesterase inhibitory activity.In addition,compounds 1 and 3-5 exhibitedα-glucosidase inhibitory activity.

关 键 词：木麻黄 凋落物 化学成分 细胞毒活性 α-糖苷酶抑制活性 乙酰胆碱酯酶抑制活性 

分 类 号：R284.2[医药卫生—中药学]