GC-MS/MS法快速测定卷烟主流烟气中苯并[a]芘的含量  

Determination of benzo[a]pyrene in the mainstream cigarette smoke by GC-MS/MS

作　　者：蔡洁云 王惠平 陈新瑞 李雪梅 刘冰 张庆刚 CAI Jie-yun;WANG Hui-ping;CHEN Xin-rui;LI Xue-mei;LIU Bin;ZHENG Qing-gang(Department of Cigarette Smoke Studies,Yunnan Tobacco Quality Supervision&Test Station,Kunming 650106,China)

机构地区：[1]云南省烟草质量监督检测站烟气室,云南昆明650106

出　　处：《云南民族大学学报（自然科学版）》2018年第2期95-101,共7页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：国家自然科学基金(81402828);中国烟草总公司云南省公司科技计划项目(2017YN31);云南省地方标准制修订项目(DBZD017-2017)

摘　　要：建立了一种使用GC-MS/MS法测定卷烟主流烟气中苯并[a]芘含量的方法,以内标法定量.将捕集了20支卷烟主流烟气的总粒相物的剑桥滤片用40 mL含有内标物的环己烷振荡萃取,过滤后检测.苯并[a]芘的检出限、定量限分别可达0.056 4 ng/cig和0.094 0 ng/mL,线性相关系数达0.999 4;日内重复性和日间重现性分别为2.39%和3.97%;在3种不同添加浓度下,加标回收率分别在93.00%~109.00%之间.该方法操作简便、重复性好、分析通量大、受环境和人为因素干扰较小,可以作为一种准确、快速测定卷烟主流烟气中苯并[a]芘含量的改进方法加以推广.对无烟气烟草制品质量控制具有重要意义.It is meaningful to determine the levels of benzo[a]pyrene in the mainstream cigarette smoke accurately and rapidly for the control of the product quality.This research adopts an inter-standard quantitative method for the determination of benzo[a]pyrene in the mainstream cigarette smoke by GC-MS/MS.By collection TPM of 20 cigarettes by a CFP and extraction by 40 mL cyclohexane,the LODs and the LOQs of BaP could achieve 0.056 4 ng/cig and 0.094 0 ng/mL,respectively,while the linear correlation efficiency is 0.999 4.The intra-assay and inter-assay precisions measured by 15 repeat runs for the BaP are 2.39%and 3.97%,respectively.And with three different added-levels,the recovery rates for all PAHs lie between 93.00%and 109.00%.It has proved that the developed method is environmental friendly,high sensitive and selective.

关 键 词：苯并[A]芘 卷烟主流烟气 GC-MS/MS法 方法优化 

分 类 号：O657.7[理学—分析化学]