氧化石墨烯修饰再生碳纤维微电极的新方法  

A New Method for Regeneration of Carbon Fiber Microelectrodes by Modification with Graphene Oxide

作　　者：程寒[1,2] 李元[1] 李梅[1] 戴梦 谷飞[1] 鲍昌昊 黄蓉萍[1] 马静芳[1] Cheng Han;Li Yuan;Li Mei;Dai Meng;Gu Fei;Bao Changhao;Huang Rongping;Ma Jingfang;Cheng Han;Li Yuan;Li Mei;Dai Meng;Gu Fei(College of Pharmaceutical Sciences,South.Central University for Nationalities,Wuhan 430074,China;National Demonstration Center for Experimental Ethnopharmacology Education,South.Central University for Nationalities,Wuhan 430074,China)

机构地区：[1]中南民族大学药学院,武汉430074 [2]民族药学国家级实验教学示范中心中南民族大学,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2018年第1期20-24,共5页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：国家自然科学基金资助项目(21205144);中央高校基本科研业务费专项资金资助项目(CZZ16004);中南民族大学学术团队资助项目(XTZ15013);中南民族大学大学生创新创业训练计划(201710524029)

摘　　要：采用电沉积法将氧化石墨烯修饰到已被五羟色胺(5-HT)毒化的碳纤维电极表面制得再生碳纤维电极,用循环伏安法(CV)和差分脉冲伏安法(DPV)研究了多巴胺(DA)在该再生电极上的电化学行为,并优化了氧化石墨烯的电沉积时间及电压.结果表明:在20 mmol/L,p H为7.2的Tris-HCl缓冲溶液中,该再生电极对多巴胺、去甲肾上腺素具有良好的电化学响应.在优化条件下,利用DPV测定,DA的氧化峰电流与其在0.1~100μmol/L呈良好的线性关系,检测下限达0.1μmol/L.Deteriorated carbon fiber microelectrodes was regenerated by graphene oxide using electrodeposition method,and the electrochemical behaviors of dopamine(DA)solution on the regenerated electrode was investigated by cyclicvoltammetry(CV)and differential pulse voltammetry(DPV).The electrodepositing time and voltage of graphene oxidewere also optimized.The results showed that in pH7.2,20mmol/L Tris?HCl buffer solution,the regenerated electrodeshowed excellent electrochemical performance for DA and NE.Under the optimized condition,the concentration of DAshowed good linear relationships with the oxidation peak current in the range of0.1～100μmol/L,with the limits ofdetection of0.1μmol/L.

关 键 词：碳纤维电极 五羟色胺 氧化石墨烯 再生电极 多巴胺 

分 类 号：O657.1[理学—分析化学]