维药野西瓜醇提物的镇痛作用研究  

Research on Analgesic Effect of the Alcohol Extract of Capparis spinosa L.

作　　者：黄先菊[1,2] 柯才华 李梅 陈慧[1] 袁琳[1] Huang Xianju;Ke Caihua;Li Mei;Chen Hui;Yuan Lin;Li Mei;Chen Hui;Yuan Lin(College of Pharmacy,South.Central University for Nationalities,Wuhan 430074,China)

机构地区：[1]中南民族大学药学院,武汉430074 [2]中南民族大学实验教学与实验室管理中心,武汉430074

出　　处：《中南民族大学学报（自然科学版）》2018年第1期46-49,共4页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：国家自然科学基金资助项目(81374064)

摘　　要：目的:研究野西瓜的镇痛作用,并探讨其作用机制.方法:体内建立福尔马林致小鼠神经性和炎症性双相疼痛、小鼠热板致痛、冰醋酸致小鼠扭体反应动物模型,观察不同实验组效果.体外以RAW264.7细胞为研究对象,探讨野西瓜醇提物对脂多糖(LPS)诱导的RAW264.7细胞释放一氧化氮(NO)的影响.结果:野西瓜醇提物对福尔马林所致小鼠双相疼痛反应呈剂量依赖性的抑制作用,使小鼠局部炎症组织前列腺素E2(PGE2)含量降低,延长小鼠热板痛阈值,减少醋酸致小鼠扭体次数,抑制RAW264.7细胞内NO的产生.结论:野西瓜醇提物有很好的抗炎镇痛效果,其作用机制之一可能是通过降低PGE2含量来发挥作用.Objective:To investigate the analgesic effect and mechanism ofCapparis spinosa L.Methods:Three painmodels including acid-induced writhing test,hot-plate test and formalin pain model in mice were used to assess the effect ofCapparis spinosa L.on nociceptive threshold in vivo.The PGE2level of the swollen hind paws induced by formalin was thendetected.The effects ofCapparis spinosa L.on nitric oxide(NO)production of RAW264.7induced by Lipopolysaccharide(LPS)in vitro.Results:Capparis spinosa L.caused significant decrease in pain responses in the first and second phases offormalin test with altering the PGE2production in the hind paw of the mice,increased the pain threshold in hot plate test,inhibited acetic acid induced writhing response,and inhibited NO production of RAW264.7.Conclusion:Capparis spinosaL.has analgesic effects,and the analgesic function may be related to its suppression of PGE2.

关 键 词：野西瓜 镇痛作用 一氧化氮 前列腺素E_(2) 

分 类 号：Q438[生物学—生理学] R965[医药卫生—药理学]