美洲大蠊提取液对慢性难愈合创面Ⅰ/Ⅲ型胶原蛋白调控研究  

Study of American Cockroach Extraction Solution in Regulating the TypeⅠ/Ⅲ Collagen of Chronic Wound

作　　者：李文华[1] 伍伟明[1] 刘筱[1] 杨敏 周雯婷 曾娟妮[2] LI Wenhua;WU Weiming;LIU Xiao;YANG Ming;ZHOU Wenting;ZENG Juanni(Hunan University of Chinese Medicine,Changsha,Hunan 410208,China;The Second Affiliated Hospital of Hunan University of Chinese Medicine,Changsha,Hunan 410005,China)

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南中医药大学第二附属医院,湖南长沙410005

出　　处：《湖南中医药大学学报》2018年第3期250-253,共4页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学基金项目(81503585)

摘　　要：目的观察美洲大蠊提取液对大鼠慢性难愈合创面组织中Ⅰ/Ⅲ型胶原蛋白的表达,探讨其促愈机制。方法将48只SPF大鼠随机分为急性创面对照组,慢性创面模型组、贝复济阳性组及美洲大蠊实验组,药物干预5、7、11 d后分批留取创面组织并处死,运用PCR方法测定创面组织中Ⅰ/Ⅲ型胶原蛋白m RNA的表达。结果Ⅰ、Ⅲ型胶原在各组中的表达趋势曲线一致,第5天,对照组表达最高,模型组最低,从第5天开始,美洲大蠊实验组中Ⅰ、Ⅲ型胶原中表达呈上升趋势并高于阳性组,第7天达到高峰后逐渐稳定下降,第11天略低于阳性组(P<0.01)。结论美洲大蠊提取液能有效提高大鼠难愈合创面前中期Ⅰ/Ⅲ型胶原蛋白的表达从而促进创面愈合,并在后期能有效降低胶原蛋白的过度表达而减少瘢痕形成。Objective To observe the effect of American Cockroach extraction solution on the expression of type玉/芋collagen in tissue of the chronic wound in rats,and to investigate the healing mechanism.Methods The 48 SPF rats were randomly divided into the acute wound control group(control group),the chronic wound model group(model group),the positive group and the experimental group.The research group kept a part of wound tissue in batches on 5th,7th,11th day after drug intervention and executed the whole rats finally.Expression quantity of type collagen玉/芋mRNA in wound tissue was measured by polymerase chain reaction(PCR).Results The curves of expression variation of collagen type I and芋were consistent in each group.On the 5th day,the expression quantity in the control group was highest and the model group's was lowest.Collagen type I/芋of the experimental group was on the rise from the 5th day and it was higher than that of positive group,it kept steadily after reaching the peak on the 7th day,and it was slightly lower than that of the positive group on 11st day(P<0.01).Conclusion American cockroach extraction solution could improve the expression of type玉/芋collagen of skin tissue in the middle time of the chronic wound healing of rats effectively,and it could reduce the excessive expression of collagen effectively so as to reduce the scar formation.

关 键 词：美洲大蠊提取液 慢性难愈合创面 Ⅰ型胶原蛋白 Ⅲ型胶原蛋白 

分 类 号：R285.5[医药卫生—中药学] R393[医药卫生—中医学]