补阳还五汤超微饮片对局灶性脑缺血大鼠神经干细胞移植后Bcl-2、Bax表达的影响  

Effects of Buyang Huanwu Ultra-Fine Powder on Apoptosis Gene Bcl-2 and Bax of Transplanted Neural Stem Cells in Rats with Focal Cerebral Ischemia

作　　者：廖亮英[1] 蔡光先[2] 周赛男[2] LIAO Liangying;CAI Guangxian;ZHOU Sainan(The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha,Hunan 410007,China;Hunan University of Chinese Medicine,Changsha,Hunan 410208,China)

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]湖南中医药大学,湖南长沙410208

出　　处：《湖南中医药大学学报》2018年第3期257-260,共4页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学基金(81302953);湖南省中医药科研计划项目(2014136);湖南省教育厅科研项目(15C1050);中药粉体与创新药物省部共建国家重点实验室培育基地开放基金(ZYFT201510)

摘　　要：目的探讨补阳还五汤超微饮片对局灶性脑缺血大鼠神经干细胞移植后凋亡相关基因Bcl-2、Bax的影响。方法将SD大鼠80只,雌雄各半,复制局灶性脑缺血大鼠模型,造模后按神经功能缺损评分进行分层,随机分为5组,每组15只,分别于移植后7 d、14 d、28 d进行神经功能评分,采用补阳还五汤超微饮片与传统饮片予以干预,于相应时间点处死大鼠后采用免疫组化法检测凋亡基因Bcl-2、Bax。结果补阳还五汤能显著减轻神经功能缺损,Bcl-2、Bax在各时间点(7 d、14 d、28 d)表达与模型组比较差异有统计学意义(P<0.05),模型加移植加补阳还五汤超微饮片组上调Bcl-2、拮抗Bax表达的作用优于模型加移植加补阳还五汤传统饮片组,差异有统计学意义(P<0.05)。结论补阳还五汤可调节脑缺血后Bcl-2、Bax的表达,是其促进神经功能恢复的可能机制之一,且超微饮片组的作用较传统饮片组更加显著。Objective To investigate the effects of Buyang Huanwu ultra-fine powder on apoptosis protein of Bcl-2 and Bax in transplanted neural stem cells in rats with focal cerebral ischemia.Methods Eighty Sprague-Dawley rats,half male and half female,were copyed the focal cerebral ischemia models,which were randomly divided into five groups,15 rats in each group.The model rats were intervened by using Buyang Huanwu ultra-fine powder and traditional decoction.At 7 d,14 d and 28 d of post-tansport,the neural function defect scale was measured.The rats were sacrified at according time.Bcl-2 and Bax were detected by immunohistochemical staining.Results Buyang Huanwu decoction could improve the neurological defect.Compared with model group,the expression of Bcl-2 and Bax increased obviously at different time points(7 d,14 d,28 d),the differnces were statistically significant(P<0.05).The model-transplantation-Buyang Huanwu ultra-fine powder group in increasing Bcl-2 and decreasing Bax was better than the model-transplantation-traditional Buyang Huanwu decoction,the difference was statistically significant(P<0.05).Conclusion Buyang Huanwu decoction could regulate the expression of Bcl-2 and Bax protein after cerebral ischemia,which may be one of mechnisms of promoting the recovery of neural function.The effect of Buyang Huanwu ultra-fine powder was better than traditional decoction.

关 键 词：补阳还五汤 局灶性脑缺血 神经干细胞 细胞移植 Bcl-2 Bax 

分 类 号：R289.3[医药卫生—方剂学] R255.2[医药卫生—中药学]