氟化钠诱导人成骨细胞凋亡相关microRNA差异表达及分析  

Differential expression of microRNAs related to apoptosis in human osteoblasts induced by sodium fluoride

作　　者：邓强[1] 张亚楼 周杨俊杰 马创[1] 盛伟斌[1] Deng Qiang;Zhang Ya-lou;Zhou Yangjunjie;Ma Chuang;Sheng Wei-bin(Department of Orthoepdics,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830011,Xinjiang Uygur Autonomous Region,China;2Department of Histology and Embryology,Preclinical Institute of Xinjiang Medical University,Urumqi 830011,Xinjiang Uygur Autonomous Region,China)

机构地区：[1]新疆医科大学第一附属医院骨科中心,新疆维吾尔自治区乌鲁木齐市830011 [2]新疆医科大学基础医学院组织胚胎学教研室,新疆维吾尔自治区乌鲁木齐市830011

出　　处：《中国组织工程研究》2018年第8期1149-1154,共6页Chinese Journal of Tissue Engineering Research

基　　金：新疆维吾尔自治区自然科学基金(2015211C040)

摘　　要：背景:长期过量摄入氟,尤其是通过饮水,可以引起慢性氟中毒骨病。该病可以导致骨骼损伤和畸形,且难以恢复。目前还没有早期诊断该病的无创或微创方法。目的:观察人成骨细胞在过量氟作用下凋亡相关mi RNA表达的情况。方法:体外培养人成骨细胞建立染氟模型,用20 mg/L Na F和40 mg/L NaF分别处理24/48 h后用PCR芯片法测定凋亡相关mi RNA的表达情况。结果与结论:(1)Na F处理24 h后发现成骨细胞内有48条mi RNA表达上调,4条mi RNA表达下调;Na F处理48 h有21条miRNA表达上调,2条miRNA表达下调;两个时间点共同上调mi RNA有9条,共同下调miRNA有1条;(2)用生物信息学软件对选出的10条人成骨细胞凋亡相关miRNA进行靶基因分析,涉及抗凋亡和促凋亡的两方面的作用,对于氟骨症的早期鉴别具有一定的理论意义。BACKGROUND:Long-term excessive intake of fluoride,especially through drinking water,can cause chronic fluorosis of bone.The disease can lead to bone damage and deformity,and is difficult to recover.Unfortunately,we have not developed a noninvasive or minimally invasive method for its early diagnosis.OBJECTIVE:To observe the expression of apoptosis-related miRNAs under the action of excessive fluorine in human osteoblasts.METHODS:The fluorine model was established in the human osteoblasts by cultured with 20 and 40 mg/L sodium fluoride for 24 and 48 hours,respectively.The expression levels of apoptosis-related miRNAs were determined by PCR array.RESULTS AND CONCLUSION:After 24-hour treatment of sodium fluoride,48 kinds of miRNAs were upregulated and 4 ones were down-regulated in the osteoblasts.After 48-hour treatment of sodium fluoride,21 kinds of miRNAs were upregulated and 2 ones were down-regulated.It showed that nine up-regulated miRNAs and one down-regulated miRNA were same in two periods.The 10 miRNAs are selected for target gene analysis on bioinformatics software that refer to the effect of anti-apoptosis and pro-apoptosis,which is of great significance for the early identification of skeletal fluorosis.

关 键 词：氟化钠 细胞凋亡 微RNAS 组织工程 

分 类 号：R318[医药卫生—生物医学工程]