不同年龄软骨组织多肽的定量分析  

Quantitative peptidomic analysis of the cartilage tissues of different ages

作　　者：周志文 鞠黎[1] 楼跃[1] Zhou Zhi-wen;Ju Li;Lou Yue(Department of Orthopedics,Children’s Hospital of Nanjing Medical University,Nanjing 210008,Jiangsu Province,China)

机构地区：[1]南京医科大学附属南京儿童医院骨科,江苏省南京市210008

出　　处：《中国组织工程研究》2018年第8期1178-1183,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:大分子量蛋白质在软骨组织的生理作用已经研究很多,但小分子量的多肽却很少涉及。目的:定量分析低年龄组和高年龄组软骨组织多肽,从差异多肽中筛选出可能跟软骨发育有关的活性多肽。方法:收集6例低年龄组(小于3岁)和8例高年龄组(6-8岁)髂前上棘下端软骨组织标本,采用液相色谱-串联质谱定量分析不同年龄组软骨多肽,应用同位素二甲基标记法分析两组多肽组成的差异。结果与结论:(1)鉴定了588个多肽在低年龄组和高年龄组软骨组织中差异表达,来源于428种蛋白质;(2)16个多肽在高年龄组中高表达(大于4倍),6个多肽在低年龄组中高表达(大于4倍);(3)通过差异多肽的分子质量、等电点和基因本体分析,初步了解了软骨组织多肽的分子学特征,为进一步寻找软骨组织活性多肽提供了信息。BACKGROUND:There are many studies on the physiological roles of high-molecular-weight protein in cartilage tissue,but low-molecular-weight peptides are rarely investigated.OBJECTIVE:To conduct a quantitative analysis of cartilage tissue peptide in low-and high-age population,and to screen active peptides related to cartilage development from the differential peptides.METHODS:The cartilage tissue samples from six cases of low age(<3 years old)and eight cases of high age(6-8 years old)population were collected,cartilage peptides analyzed quantitatively by liquid chromatography tandem mass spectrometry,and the differences in the peptide composition between two groups were analyzed by isotope dimethyl labeling method.RESULTS AND CONCLUSION:We identified 588 differential peptides in the cartilage tissues of two groups,which were originated from 428 proteins.Sixteen peptides were present at higher levels in the high-age group(over 4-fold expression),and 6 were present at higher levels in the low-age group(over 4-fold expression).Through analyzing the molecular mass,isoelectric point and gene ontology of the differential peptides,we preliminarily understand the characteristics of cartilage peptides at molecular level,which provides a new perspective for searching more active cartilage peptides.

关 键 词：软骨 肽类 年龄组 组织工程 

分 类 号：R318[医药卫生—生物医学工程]