依达拉奉干预脊髓损伤模型大鼠Ⅰ型和Ⅳ型胶原蛋白的表达  

Edaravone effects on the expression levels of collagen type Ⅰ and Ⅳ after spinal cord injury in rats

作　　者：李仁斌 余光书 林焱斌 周家烽 黄奕祺 郑伟 Li Ren-bin;Yu Guang-shu;Lin Yan-bin;Zhou Jia-feng;Huang Yi-qi;Zheng Wei(Department of Orthopedics,Fuzhou Second Hospital Affiliated to Xiamen University,Fuzhou 350007,Fujian Province,China)

机构地区：[1]厦门大学附属福州第二医院骨科,福建省福州市350007

出　　处：《中国组织工程研究》2018年第8期1235-1240,共6页Chinese Journal of Tissue Engineering Research

基　　金：福州市科技局社会发展项目(2016-S-123-16);福建省卫生计生中青年骨干人才培养项目(2017-ZQN-73)~~

摘　　要：背景:依达拉奉是一种有效的氧自由基清除剂,有研究报道其可以有效改善脊髓损伤后肢体运动功能,但是其对损伤组织修复的作用机制尚不明确。目的:通过观察脊髓损伤后大鼠肢体功能评分的变化及Ⅰ型胶原蛋白和Ⅳ型胶原蛋白表达情况,探讨依达拉奉促进脊髓损伤大鼠肢体功能恢复的机制。方法:将36只大鼠随机分为3组,每组12只:假手术组不损伤脊髓,仅去除椎板后缝合+生理盐水腹腔注射;模型组去除椎板后行NYU脊髓打击器建立脊髓损伤模型+腹腔注射生理盐水;依达拉奉组:去除椎板后行NYU脊髓打击器建立脊髓损伤模型+腹腔注射依达拉奉。造模后第1天开始进行药物干预,持续7 d。干预后1,3,5及7 d对各组大鼠进行Basso Beattie Bresnahan(BBB)肢体功能评分;干预后第7天,处死大鼠,取出脊髓,尼式染色观察脊髓神经元细胞的形态;免疫组化法和Western blot检测Ⅰ型和Ⅳ型胶原蛋白表达情况。结果与结论:(1)与模型组相比,干预第5天后,依达拉奉组的BBB评分显著升高(P<0.05);(2)尼式染色显示假手术组脊髓灰白质界限清晰,神经细胞胞质内可见较大且明显的核仁;依达拉奉组,细胞形态较为完整,细胞水肿较轻,瘀血面积较小;模型组神经元细胞空洞较多且大,细胞核肿胀,甚至出现核仁消失的情况;(3)Western blot和免疫组化实验结果显示依达拉奉组的Ⅰ型胶原蛋白和Ⅳ型胶原蛋白表达水平显著高于模型组多(P<0.05);(4)结果提示,依达拉奉对脊髓损伤后大鼠的肢体功能障碍有显著改善作用,可能与增加Ⅰ型胶原蛋白和Ⅳ型胶原蛋白的表达有关。BACKGROUND:Edaravone,an effective free radical scavenger,has been reported to significantly improve the rehabilitation of limb locomotion after spinal cord injury(SCI),but the underlying mechanism remains unclear.OBJECTIVE:To explore the mechanism underlying edaravone promoting the recovery of limb locomotion in rats with SCI by observing the Basso,Beattie,Bresnahan scores and expression levels of collagen type I and IV.METHODS:Thirty-six rats were randomly allocated into three groups(n=12 per group):sham group(laminectomy plus intraperitoneal injection of normal saline),model group(SCI model by NYU impactor plus intraperitoneal injection of normal saline),and edaravone group(SCI model by NYU impactor plus intraperitoneal injection of edaravone).All rats were given the administration at the 1st day post-SCI for consecutive 7 days.The Basso,Beattie,Bresnahan scores were tested at 1,3,5 and 7 days post treatment.On day 7,all rats were sacrificed to remove the spinal cord,and the morphology of neurons in the spinal cord were observed by Nissl staining;the expression levels of collagen type I and IV were detected by immunohistochemistry and western blot assays.RESULTS AND CONCLUSION:Compared with the model group,the Basso,Beattie,Bresnahan scores in the edaravone group were significantly increased at day 5 post treatment(P<0.05).Nissl staining showed a clear boundary between grey matter and white matter,and a large nucleolus in the neurocytoplasm in the sham group;there was a complete structure of neurons,slight cellular swelling and small hematoma area in the edaravone group;many and large cavitations and swollen nucleus were found in the neurons,even without nucleolus.Immunohistochemistry and western blot assay results showed that the expression levels of collagen type I and IV in the edaravone group were significantly higher than those in the model group(P<0.05).These results indicate that edaravone can promote the recovery of limb locomotion of rats with SCI,probably via up-regulating the expression levels of col

关 键 词：脊髓损伤 模型 动物 胶原I型 组织工程 

分 类 号：R318[医药卫生—生物医学工程]