秀丽莓中鞣花酸的含量测定和抗氧化活性评测及对接研究  

Content determination,antioxidant activity estimation and docking study of ellagic acid in Rubus amabilis

作　　者：刁玉林[1,2] 朱冬青 刘斌 DIAO Yulin;ZHU Dongqing;LIU Bin(School of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100102,China;National Engineering and Technology Research Center of Chirality Pharmaceutical,Shandong Lunan Pharmaceutical Group Limited Company,Linyi 273400,China)

机构地区：[1]北京中医药大学中药学院,北京100102 [2]国家手性制药工程技术研究中心,山东鲁南制药股份有限公司,临沂273400

出　　处：《西北药学杂志》2018年第1期1-5,共5页Northwest Pharmaceutical Journal

基　　金：国家自然科学基金项目(编号:81260684);北京中医药大学自主选题基金项目(编号:2013-jybzzxs-113)

摘　　要：目的建立秀丽莓中鞣花酸的含量测定方法并对其抗氧化活性进行研究。方法采用RP-HPLC法,SunFireTMC18色谱柱(150mm×4.6mm,5μm),乙腈-4mL·L-1磷酸水(20∶80)为流动相;流速:1.0mL·min-1;检测波长:254nm;柱温:35℃。有机结合计算机分子模拟及体外DPPH·抗氧化活性评价方法对其可能的作用机制及活性进行深入探讨。结果鞣花酸的线性范围为0.081 92~0.409 60μg(r=0.999 7,n=6);平均回收率(n=6)为99.51%(RSD=2.00%)。该成分可通过结构中的酚羟基经氢键、苯环经π-π键作用与受体蛋白的氨基结合起效,具有较维生素C更强的抗氧化活性。结论该方法可迅速、准确地测定秀丽莓中的鞣花酸含量,可作为该药材中主要抗氧化成分鞣花酸质量控制和评价的有效方法,为该药材的进一步开发提供判断方法。To establish a method to determine the content and to estimate the antioxidant activity of ellagic acid in Rubus amabilis.The RP-HPLC separation was performed on a SunFire TM Ci8 analytical column(150 m m X4.6 mm,5 jum).The mobile phase was acetonitrile-water containing 4 mL.L 1 H 3 P 0 4(20:80)with a flow rate of 1.0 mL.min 1.The detection wavelength was set at 254 nm,and the column temperature was 35°C.The computer molecular simulation and 2,2/-diphenyl-l-picrylhydrazyl(DPPH.)antioxidation in vitro methods were used together to estimate the antioxidant activity of ellagic acid.The liner range of ellagic acid was 0.081 92-0.409 60 p.g(r=0.999 7,n=6).The average recovery(n=6)was 99.51%(RSD=2.0 0%).Ellagic acid was functioned at the-N H 2 groups in receptor proteins by the hydrogen bonds between-OH groups and tc-tc bonds among benzene rings,which resulted in the more significant antioxidant activity with vitamin C.The method is simple,stable and accurate with a good reproducibility,and can be used as a method for quality control and evaluation of the main antioxidation component,ellagic acid,in Tibetan medicine Rubus amabilis.The method can be used in the quality evaluation of the further development of the plant.

关 键 词：秀丽莓 鞣花酸 含量测定 分子模拟 抗氧化 

分 类 号：R917.[医药卫生—药物分析学]