热水提取与超声提取的麦冬多糖结构与构象特征的比较研究  

A Comparative Study on Structure and Conformation of Polysaccharides in Ophiopogon Japonicus by Hot Water and Ultrasonic Extraction

作　　者：王小梅 王妙 薛红杰 张明 郝大鹏 孙润广[2] WANG Xiao-mei;WANG Miao;XUE Hong-jie;ZHANG Ming;HAO Da-peng;SUN Run-guang(Science College,Xi an Aeronautical University,Xi an 710077,China;Laboratory of Biophysics and Biomedicine Engineering,College of Physics and Information Technology,Shaanxi Normal University,Xi an 710062,China)

机构地区：[1]西安航空学院理学院,陕西西安710077 [2]陕西师范大学物理学与信息技术学院生物物理与生物医学工程实验室,陕西西安710062

出　　处：《分析测试学报》2018年第1期23-30,共8页Journal of Instrumental Analysis

基　　金：西安航空学院校级项目(2016KY1215)

摘　　要：利用热水与超声提取麦冬粗多糖,DEAE-cellulose52纤维素与Sephacryl S-300凝胶柱层析对其进行分离纯化,得到热水提取的麦冬多糖均一组分WPOJ-DS与超声提取的麦冬多糖均一组分UPOJ-DS。采用高效液相色谱法、气相色谱法、红外光谱法、部分酸水解、甲基化分析及核磁共振对WPOJ-DS与UPOJDS的结构进行表征;利用刚果红实验、圆二色谱实验及原子力显微镜对其溶液构象进行了比较研究。结果表明,超声提取对麦冬多糖的分子量、单糖组成摩尔比及构型会产生影响,WPOJ-DS与UPOJ-DS的主链均主要由→6)-D-Glcp(1→和→3,6)-D-Glcf(1→组成,但WPOJ-DS中存在→6)-β-D-Glcp(1→,而UPOJDS中没有;刚果红实验、圆二色谱实验与原子力显微镜结果显示UPOJ-DS存在螺旋结构,而WPOJ-DS没有;且在不同溶液环境中,WPOJ-DS与UPOJ-DS的溶液构象不同。In this paper,ophiopogon japonicus crude polysaccharide was extracted by hot water and ultrasonic extraction,then purified by DEAE-cellulose52 cellulose column chromatography and Sephacryl S-300 gel column chromatography.Homogeneous polysaccharide WPOJ-DS extracted with hot water and UPOJ-DS extracted by ultrasound were obtained.The structures of WPOJ-DS and UPOJ-DS were characterized by high performance liquid chromatography,gas chromatography,infrared spectroscopy,partial acid hydrolysis,methylation analysis and magnetic resonance imaging.The solution conformation of WPOJ-DS and UPOJ-DS were studied by congo red test,circular dichroism spectra and atomic force microscopy.The results showed that there were differences between WPOJ-DS and UPOJ-DS,which existed in the molecular weight,molar ratio of monosaccharide and the solution conformation,while the differences also demonstrated that the structure characterization and solution conformation could be effected by ultrasonic extraction.The main chains in both WPOJ-DS and UPOJ-DS mainly composed of→6)-D-Glcp(1→and→3,6)-D-Glcf(1→.The structure of→6)-β-D-Glcp(1→existed in WPOJ-DS but not in UPOJ-DS.Congo red experiment,circular dichroism spectra and chromatography and atomic force microscopy showed that there was a helix structure in UPOJ-DS but not in WPOJ-DS.In addition,the solution conformations of WPOJ-DS and UPOJ-DS were different in different environment solutions.

关 键 词：麦冬多糖 超声提取 结构 溶液构象 原子力显微镜 

分 类 号：O655.4[理学—分析化学]