毛细管电泳-间接化学发光法检测尿液与血液中吗啡含量  

Determination of Morphine in Urine and Blood by Capillary Electrophoresis Coupled with Indirect Chemiluminescence Detection

作　　者：朱怀娇 韩燕祯 杜鹏男 康凯 毕思远 徐向东[1] ZHU Huai-jiao;HAN Yan-zhen;DU Peng-nan;KANG Kai;BI Si-yuan;XU Xiang-dong(School of Public Health,Hebei Medical University,Shijiazhuang 050017,China;Shenzhen Bioeasy Biotechnologies Co.,Ltd.,Shenzhen 518101,China)

机构地区：[1]河北医科大学公共卫生学院,河北石家庄050017 [2]深圳市易瑞生物技术有公司,广东深圳518101

出　　处：《分析测试学报》2018年第1期108-111,共4页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(81402723);深圳市科技计划项目-食品安全快速检测技术中心(GCZX20140509163151273)

摘　　要：在碱性溶液中,吗啡对Ag(Ⅲ)配合物-鲁米诺化学发光体系的化学发光信号有显著抑制作用,且抑制程度与吗啡浓度成正比。基于此,建立了毛细管电泳-间接化学发光检测尿液和血液中吗啡含量的方法。在优化条件下,方法检出限为0.75 mg/L,线性范围为2.0~30.0 mg/L,相关系数(r)为0.999 8;对20mg/L的吗啡进行7次平行测定,测得的相对标准偏差(RSD)为2.0%。结合固相萃取法,该方法成功用于尿液和血液中吗啡含量的测定,平均加标回收率分别为109.0%与101.3%。该方法分辨率高、灵敏、分析成本低,可用于尿液和血液中吗啡含量的测定。Based on the fact that morphine has a significant inhibitory effect on Ag(Ⅲ)complex-luminol chemiluminescence system in alkaline solution and the effect is proportional to the concentration of morphine,a novel method was established for the determination of morphine by capillary electrophoresis coupled with indirect chemiluminescence detection.Under the optimized conditions,there was a linear range of 2.0-30.0 mg/L for morphine with a correlation coefficient(r)of 0.999 8 and the detection limit of 0.75 mg/L.The RSD(n=7)was 2.0%for morphine in a concentration of 20 mg/L.Combined with solid phase extraction,the method was successfully applied in the detection of morphine in urine and blood samples with the average recoveries of 109.0%and 101.3%,respectively.With the advantages of high-resolution,sensitivity and low-cost,the method could be used for the determination of morphine in urine and blood samples.

关 键 词：化学发光 毛细管电泳 吗啡 固相萃取 尿液 血样 

分 类 号：O657.8[理学—分析化学]